β-羥丁酸(bHB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB)
OHB; BHBA; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid; β-Hydroxybutyric acid
- 編號KSB022Ge11
- 物種Pan-species (General,通用)相同的名稱,不同的物種。
- 試劑內(nèi)容包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍1.23-100ug/mL
- 可適靈敏度0.48ug/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 8726 ¥ 14544¥ 26179¥ 50904¥ 87264
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特異性
試劑盒中的抗體對β-羥丁酸(bHB)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Pan-species (General,通用) | 應用(僅供研究使用,不用于臨床診斷!) |
CPB022Ge11 | β-羥丁酸(bHB)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPB022Ge21 | β-羥丁酸(bHB)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAB022Ge01 | β-羥丁酸(bHB)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEB022Ge | β-羥丁酸(bHB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB022Ge | β-羥丁酸(bHB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB022Ge11 | β-羥丁酸(bHB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression[Endocrine:Source] |
Chem Commun (Camb) | A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitro[PubMed: 25846235] |
PLoS One | Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine[pubmed:27501393] |
Buffalo Bulletin | Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes[] |
Journal of Integrative Agriculture | Temporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows[] |