連環(huán)蛋白β1(β-catenin)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Beta Catenin (β-catenin)
CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein
- 編號(hào)KSB021Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍15.6-1000pg/mL
- 可適靈敏度6.7pg/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)連環(huán)蛋白β1(CTNNb1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
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參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinica Chimica Acta | Versican and its associated molecules: Potential diagnostic markers for multiple myeloma[Pubmed:25623955] |
| Journal of Bone and Mineral Research | Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth[Pubmed:25639729] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats[PubMed: 26396666] |
| Scientific Reports | Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA.[pubmed:28533512] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618] |
| Scientific Reports | Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA[Pubmed: 28533512] |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer[Doi: 10.15369/sujms.30.359] |
| Advances in?Medical?Sciences | Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney?[Pubmed: 30738287] |
| Biomed Pharmacother | Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway[Pubmed: 32028241] |
| Journal of Cosmetic Dermatology | Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling?…[Pubmed: 33179848] |
| Nutrients | Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia[Pubmed: 32784915] |
| Oncotarget | Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme[33473259] |
| Cytokine | QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model[34564023] |