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窖蛋白(CAV1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Caveolin 1 (CAV1)

VIP21; Caveolae Protein 22kDa

  • 窖蛋白(CAV1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 窖蛋白(CAV1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)窖蛋白(CAV1)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA214Hu01窖蛋白(CAV1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CPA214Hu21窖蛋白(CAV1)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
PAA214Hu08窖蛋白(CAV1)多克隆抗體WB; IHC; ICC; IP.
PAA214Hu01窖蛋白(CAV1)多克隆抗體WB; IHC; FCM
MAA214Hu22窖蛋白(CAV1)單克隆抗體WB; IHC; ICC; IP.
SEA214Hu窖蛋白(CAV1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA214Hu窖蛋白(CAV1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA214Hu01窖蛋白(CAV1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
Mendel NetNovel prostate cancer tumour markers in a cell line model[Mendelu: source]
Oncology reportsCaveolin-1 as a potential high-risk prostate cancer biomarker[PubMed: 22159333]
Anticancer ResearchIncreased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese men[Pubmed: 23645736]
Sarcoidosis Vasc Diffuse Lung Dis.?Decrased sputum caveolin-1 is associated with systemic sclerosis related lung disease[Pubmed:24751454]
Cancer Genomics and ProteomicsSignificant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression[PubMed: 26543085]
AtherosclerosisGenomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction[Pubmed:26775120]
Arthritis Research & TherapyIncreased caveolin-1 in intervertebral disc degeneration facilitates repair[Pubmed:26939667]
Oxid Med Cell LongevLower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic Stroke[Pubmed:27119011]
InflammationCaveolin-1?Promotes?the?Imbalance?of?Th17/Treg?in?Patients?with?Chronic?Obstructive?PulmonaryDisease.[pubmed:27613621]
Oxidative Medicine and Cellular LongevitySwim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis Mice[Pubmed:29849903]
MicrocirculationEndothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced?…[Pubmed:29665152]
Genetic Testing and Molecular BiomarkersThe CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic?…[]
Cell RepSIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR|?[34077730]
researchsquareCav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA??-[]
Molecules5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein[Pubmed:35566232]