皮質(zhì)醇(Cor)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Cortisol (Cor)
Hydrocortisone
- 編號(hào)KSA462Ge11
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, urine and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍68.59-50,000 pg/m
- 可適靈敏度24.58pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)皮質(zhì)醇(Cor)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA462Ge11 | 皮質(zhì)醇(Cor)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA462Ge21 | 皮質(zhì)醇(Cor)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA462Ge01 | 皮質(zhì)醇(Cor)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA462Ge02 | 皮質(zhì)醇(Cor)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA462Ge81 | 皮質(zhì)醇(Cor)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA462Ge71 | 皮質(zhì)醇(Cor)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA462Ge21 | 皮質(zhì)醇(Cor)單克隆抗體 | IHC |
| MAA462Ge22 | 皮質(zhì)醇(Cor)單克隆抗體 | ELISA |
| MEA462Ge | 皮質(zhì)醇(Cor)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA462Ge | 皮質(zhì)醇(Cor)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA462Ge | 皮質(zhì)醇(Cor)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA462Ge | 皮質(zhì)醇(Cor)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA462Ge | 皮質(zhì)醇(Cor)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA462Ge | 皮質(zhì)醇(Cor)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA462Ge11 | 皮質(zhì)醇(Cor)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Research in Veterinary Science | Use of infrared thermography to assess the influence of high environmental temperature on rabbits[ScienceDirect: S0034528813001380] |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han Chinese[Pubmed:24124699] |
| Theriogenology | Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model[Pubmed:25442388] |
| Animal Production Science | Effect of high temperatures on breeding rabbit behaviour[Publish:Source] |
| JBCD | Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students[Index.Php: Jbcd] |
| International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats[PubMed: 26684638] |
| IET Micro & Nano Letters | Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chip[xpls:7393900] |
| Optoelectronics Letters | Fabrication and characterization of SPR chips with the modified bovine serum albumin[article:10.1007] |
| Journal?of?Dairy?Science | Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves[publication:306322503] |
| Acta Veterinaria Hungarica | Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation[acve-2017-0006] |
| BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?…[Pubmed:29540178] |
| Indian Journal of Animal Sciences | Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition[:] |
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| International Journal of Current Microbiology and Applied Sciences | Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons[] |
| Journal of Animal & Plant Sciences | A study on certain blood biomarkers of pregnant Cholistani cows.[] |
| Animals | The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot Study[Pubmed: 30866503] |
| Medicine | Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexia[Pubmed: 32028434] |
| MICROBIAL PATHOGENESIS | Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirus[Pubmed: 32926999] |
| AQUACULTURE RESEARCH | Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition[] |
| AQUACULTURE RESEARCH | Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense[] |
| Anti-stress activity of some plants extracts of the North Caucasus flora[] | |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves[33610512] |
| Parasit Vectors | Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle[33722267] |
| J Am Heart Assoc | Post?\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest[33960200] |
| Animals | The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets[33435211] |
| Preventive Veterinary Medicine | Stress response after race and endurance training sessions and competitions in Arabian horses[33497894] |
| Current Psychology | Associations of Chinese social face with cortisol level and glucocorticoid receptor gene[] |
| Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1?…[] |