半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- 編號KSJ322Ra01
- 物種Rattus norvegicus (Rat,大鼠)相同的名稱,不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.119ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 6149 ¥ 10248¥ 18446¥ 35868¥ 61488
- 欲了解實際交易價格和更多情況,請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對半胱氨酸蛋白酶抑制劑B(CSTB)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體 | WB; IHC; ICC; IP. |
LAJ322Ra71 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAJ322Ra24 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB |
MAJ322Ra26 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB |
MAJ322Ra22 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAJ322Ra21 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB; IHC |
LAJ322Ra74 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEJ322Ra | 半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMJ322Ra | 半胱氨酸蛋白酶抑制劑B(CSTB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
AIDS. | Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders[PubMed: 23291538] |
International Journal of Circumpolar Health | Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer[PubMed: PMC3754495] |
PLoS One. | Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis[PubMed: PMC3365072] |
Journal of Neuroimmune Pharmacology | Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages[Pubmed:25209871] |
European Journal of Cancer Supplements | A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system[Science: Article] |
Annals of Clinical Biochemistry | Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.[pubmed:28766361] |