細胞色素P450家族成員2E1(CYP2E1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Cytochrome P450 2E1 (CYP2E1)
CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1
- 編號KSA988Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.064ng/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對細胞色素P450家族成員2E1(CYP2E1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA988Ra01 | 細胞色素P450家族成員2E1(CYP2E1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA988Ra02 | 細胞色素P450家族成員2E1(CYP2E1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA988Ra02 | 細胞色素P450家族成員2E1(CYP2E1)多克隆抗體 | WB; IHC |
| PAA988Ra01 | 細胞色素P450家族成員2E1(CYP2E1)多克隆抗體 | WB,IHC,ICC/IF |
| LAA988Ra71 | 細胞色素P450家族成員2E1(CYP2E1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA988Ra21 | 細胞色素P450家族成員2E1(CYP2E1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA988Ra | 細胞色素P450家族成員2E1(CYP2E1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA988Ra | 細胞色素P450家族成員2E1(CYP2E1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA988Ra01 | 細胞色素P450家族成員2E1(CYP2E1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
| 雜志 | 參考文獻 |
| BMC Complementary and Alternative Medicine | Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats[PubMed: PMC3605171] |
| Evidence-Based Complementary Alternative Medicine | Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats[PubMed: PMC3749608] |
| BMC Complementary and Alternative Medicine | CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat[PubMed: PMC3519517] |
| Asian Journal of Biochemical and Pharmaceutical Research | Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source] |
| The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats[Pubmed:24701154] |
| Journal of Functional Foods | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo[publication:307092820] |
| CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLA | Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats.[59f7c85aaca272607e2d8d7a] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605] |
| Nutrients | Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages[Pubmed: 31935882] |
| Antioxidants | Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress[34573017] |