表皮生長(zhǎng)因子(EGF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- 編號(hào)KSA560Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍15.62-1000pg/mL
- 可適靈敏度6.3pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 5746¥ 9576¥ 17237¥ 33516¥ 57456
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)表皮生長(zhǎng)因子(EGF)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA560Ra61 | 表皮生長(zhǎng)因子(EGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA560Ra01 | 表皮生長(zhǎng)因子(EGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA560Ra01 | 表皮生長(zhǎng)因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA560Ra01 | 表皮生長(zhǎng)因子(EGF)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Ra22 | 表皮生長(zhǎng)因子(EGF)單克隆抗體 | IHC |
| MAA560Ra23 | 表皮生長(zhǎng)因子(EGF)單克隆抗體 | IHC |
| MAA560Ra25 | 表皮生長(zhǎng)因子(EGF)單克隆抗體 | IHC |
| MAA560Ra27 | 表皮生長(zhǎng)因子(EGF)單克隆抗體 | IHC |
| MAA560Ra24 | 表皮生長(zhǎng)因子(EGF)單克隆抗體 | WB; IHC |
| MAA560Ra21 | 表皮生長(zhǎng)因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA560Ra72 | 表皮生長(zhǎng)因子(EGF)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA560Ra | 表皮生長(zhǎng)因子(EGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA560Ra | 表皮生長(zhǎng)因子(EGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA560Ra | 表皮生長(zhǎng)因子(EGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA560Ra01 | 表皮生長(zhǎng)因子(EGF)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA560Ra01 | 表皮生長(zhǎng)因子(EGF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model[PubMed: 20438940] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism[PubMed: PMC3623607] |
| Plos one | The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor[PubMed: PMC3590171] |
| Diabetes. | Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity[Pubmed:25795216] |
| Eur Rev Med Pharmacol Sci.? | Association of colon adenomas and skin tags: coincidence or coexistence?[Pubmed:24763889] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell[PubMed: 26628972] |
| ACS Applied Materials & Interfaces | Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness.[pubmed:27709887] |
| Canadian Journal of Physiology and Pharmacology | The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats[pubmed:28177680] |
| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury[pubmed:28522195] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets[pubmed:28811665] |
| International Journal of?Nanomedicine | Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human?…[Pubmed: 30233172] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine[Pubmed: 33212364] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water[33428732] |
| Cell Death & Disease | Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization[34006822] |
| Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??-[33381190] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?…[Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats[] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy[] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats[] |