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雌激素受體β(ERb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Estrogen Receptor Beta (ERb)

ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2

  • 雌激素受體β(ERb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 雌激素受體β(ERb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對雌激素受體β(ERb)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA437Hu01雌激素受體β(ERb)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA437Hu01雌激素受體β(ERb)多克隆抗體WB; IHC; ICC; IP.
LAA437Hu71雌激素受體β(ERb)多克隆抗體(生物素標記)WB; IHC; ICC.
LAA437Hu81雌激素受體β(ERb)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
FAA437Hu11抗雌激素受體β(ERb)多克隆抗體(標記)FCM
MAA437Hu22雌激素受體β(ERb)單克隆抗體WB; IHC; ICC; IP.
MAA437Hu21雌激素受體β(ERb)單克隆抗體WB; IHC; ICC; IP.
SEA437Hu雌激素受體β(ERb)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA437Hu雌激素受體β(ERb)檢測試劑盒(化學(xué)發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA437Hu雌激素受體β(ERb)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA437Hu01雌激素受體β(ERb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
The Journal of Steroid Biochemistry and Molecular BiologyNeuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling[Pubmed:24846829]
Biochem Biophys Res CommunDifferential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas[PubMed: 26529545]
Mol NeurobiolSelective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia[PubMed: 26476840]
J Steroid Biochem Mol BiolNeuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling[PubMed: 24846830]
Journal of Environmental SciencesBisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts[science:S1001074216300924]
Biol Open.Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine.[pubmed:27875242]
Biomedicine & PharmacotherapyPostmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY[Pubmed:29807224]
Journal of Applied Pharmaceutical SciencePhysical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract[Pbulication:325682424]
Carleton University Research Virtual EnvironmentIn Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts[]
Life SciencesMiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl[34273377]
CardiometryModifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue[]