半乳糖凝集素3(GAL3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Galectin 3 (GAL3)
LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29
- 編號KSA303Hu01
- 物種Homo sapiens (Human,人)相同的名稱,不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.054ng/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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特異性
試劑盒中的抗體對半乳糖凝集素3(GAL3)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA303Hu01 | 半乳糖凝集素3(GAL3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA303Hu61 | 半乳糖凝集素3(GAL3)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA303Hu01 | 半乳糖凝集素3(GAL3)活性蛋白 | Cell?culture;?Activity?Assays. |
PAA303Hu01 | 半乳糖凝集素3(GAL3)多克隆抗體 | WB,IHC,ICC/IF |
FAA303Hu01 | 抗半乳糖凝集素3(GAL3)多克隆抗體 | Flow cytometry. |
LAA303Hu71 | 半乳糖凝集素3(GAL3)多克隆抗體(生物素標(biāo)記) | WB |
LAA303Hu81 | 半乳糖凝集素3(GAL3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA303Hu24 | 半乳糖凝集素3(GAL3)單克隆抗體 | IHC |
MAA303Hu23 | 半乳糖凝集素3(GAL3)單克隆抗體 | WB; IHC; ICC; IP. |
MAA303Hu22 | 半乳糖凝集素3(GAL3)單克隆抗體 | WB; IHC |
MAA303Hu21 | 半乳糖凝集素3(GAL3)單克隆抗體 | WB; IHC |
FAA303Hu02 | 抗半乳糖凝集素3(GAL3)單克隆抗體 | Flow cytometry. |
SEA303Hu | 半乳糖凝集素3(GAL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA303Hu | 半乳糖凝集素3(GAL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA303Hu | 半乳糖凝集素3(GAL3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA303Hu01 | 半乳糖凝集素3(GAL3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
Clinica Chimica Acta | Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population[Pubmed:25578395] |
Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension[PubMed: 25747153] |
Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension[PubMed: 25984599] |
Scientifi Reportcs | Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices[PubMed: 26582585] |
Clin Chim Acta | Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population[PubMed: 25578396] |
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION | Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4.[pubmed:28431936] |
昆明醫(yī)科大學(xué)學(xué)報 | 半乳糖凝集素3 在COPD 和肺癌中的表達及其意義.[2095610X] |
Archives of Medical Science | Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department?[publication:305625095] |
Biosensors & Bioelectronics | A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod.[pubmed:29096363] |
Scientific Reports | The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction.[pubmed:28827564] |
Clinica Chimica Acta | Galectin-3 in patients with coronary heart disease and atrial fibrillation[Pubmed:29287900] |
Advances in Experimental?Medicine?and?Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease[Pubmed:29429028] |
FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure[Pubmed: 30230922] |
american journal of physiology-heart and circulatory physiology | Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus[Pubmed: 32216615] |
Cardiology | Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation[Pubmed: 32516780] |
AUSTRALIAN DENTAL JOURNAL | The effect of initial periodontal treatment on gingival crevicular fluid galectin?\3 levels in participants with periodontal disease[33378559] |
Cell Death & Disease | Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis[33771973] |
Int J Clin Pract | Serum Galectin?\3 Levels in Patients with Psoriasis[34137138] |
Phytomedicine | Zerumbone, a humulane sesquiterpene from Syringa pinnatifolia, attenuates cardiac fibrosis by inhibiting of the TGF-β1/Smad signaling pathway after myocardial?…[Pubmed:35405613] |
Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?…[Pubmed:35687771] |