促生長激素釋放激素(GHRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Growth Hormone Releasing Hormone (GHRH)
GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin
- 編號KSA438Mu11
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.63pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對促生長激素釋放激素(GHRH)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA438Mu02 | 促生長激素釋放激素(GHRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA438Mu01 | 促生長激素釋放激素(GHRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA438Mu02 | 促生長激素釋放激素(GHRH)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA438Mu01 | 促生長激素釋放激素(GHRH)多克隆抗體 | WB; IHC |
| LAA438Mu81 | 促生長激素釋放激素(GHRH)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA438Mu71 | 促生長激素釋放激素(GHRH)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| CEA438Mu | 促生長激素釋放激素(GHRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA438Mu | 促生長激素釋放激素(GHRH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA438Mu11 | 促生長激素釋放激素(GHRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| 19 | Role of GHRH in Sleep and Growth Impairments Induced by Upper Airway Obstruction in Rats[Ers: 00197610] |
| 19 | Role of growth hormone-releasing hormone in sleep and growth impairments induced by upper airway obstruction in rats[PubMed: 21406516] |
| Global Veterinaria | Effect of Moringa Oleifera Extract on Nicotine Induced Neurotoxicity in Female Rat and Their Embryos[Idosi: Source] |
| Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice[Pubmed: 30404008] |
| Drug?Testing?and?Analysis | An Immuno‐PCR screen for the detection of CJC‐1295 and other Growth Hormone Releasing Hormone analogues in equine plasma[Pubmed: 30489688] |
| Sleep and Breathing | Effects of somatotropic axis on cognitive dysfunction of obstructive sleep apnea[Pubmed: 31073904] |
| Diabetes | Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus.[33980693] |
| Sci Rep | Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients[Pubmed:35508633] |