硫酸肝素蛋白聚糖(HSPG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG)
SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan
- 編號KSA565Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1000pg/mL
- 可適靈敏度5.9pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對硫酸肝素蛋白聚糖(HSPG)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)多克隆抗體 | WB; ICC/IF |
| LAA565Hu71 | 硫酸肝素蛋白聚糖(HSPG)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA565Hu81 | 硫酸肝素蛋白聚糖(HSPG)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA565Hu22 | 硫酸肝素蛋白聚糖(HSPG)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA565Hu | 硫酸肝素蛋白聚糖(HSPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA565Hu | 硫酸肝素蛋白聚糖(HSPG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA565Hu01 | 硫酸肝素蛋白聚糖(HSPG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Applied Pharmaceutical Science | Cytotoxic effects of antiglypican-3 against HepG3 cell line[Ebscohost: Source] |
| Journal of Reproductive Immunology | Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients[Pubmed: 24661950] |
| European journal of pharmacology | Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme[Pubmed: 24530413] |
| European Journal of Pharmacology | Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma[Pubmed:25449037] |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance[Pubmed:25692257] |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection[pubmed:27643669] |
| Apmis | Circulating syndecans during critical illness.[pubmed:28256016] |
| Biomedicine & Pharmacotherapy | Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and[Pubmed:29669302] |
| Drug?Delivery | Glioma targeting peptide modified apoferritin nanocage[Pubmed:29726297] |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass[Pubmed: 30687934] |
| Animals | Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …[] |
| LIFE SCIENCES | Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients[33781826] |
| Genet Mol Biol | A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate[34042151] |
| Poultry Science | Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine??-[33518110] |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo?…[34423832] |