吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO)
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
- 編號(hào)KSB547Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.131ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)吲哚胺2,3-雙加氧酶(IDO)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB547Hu01 | 吲哚胺2,3-雙加氧酶(IDO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB547Hu01 | 吲哚胺2,3-雙加氧酶(IDO)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB547Hu01 | 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體 | WB; IHC; ICC/IF |
| LAB547Hu71 | 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體(生物素標(biāo)記) | WB |
| LAB547Hu81 | 吲哚胺2,3-雙加氧酶(IDO)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAB547Hu22 | 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | WB;ICC/IF;FCM |
| MAB547Hu24 | 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB547Hu21 | 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | WB; IHC |
| MAB547Hu23 | 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB547Hu08 | 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | Flow cytometry. |
| FAB547Hu06 | 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | Flow cytometry. |
| FAB547Hu04 | 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | Flow cytometry. |
| FAB547Hu02 | 抗吲哚胺2,3-雙加氧酶(IDO)單克隆抗體 | Flow cytometry. |
| LAB547Hu72 | 吲哚胺2,3-雙加氧酶(IDO)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB547Hu | 吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB547Hu | 吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSB547Hu01 | 吲哚胺2,3-雙加氧酶(IDO)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB547Hu01 | 吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Blood | CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects[Pubmed: 21803856] |
| Clinical Transplantation | Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT[Wiley: source] |
| Indonesian Journal of Cancer Chemoprevention | Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell[Iregway: Source] |
| PLoS ONE | CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms[PubMed: PMC3683035] |
| Hepatology | Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma[Pubmed: 23960017] |
| Free Radical Biology and Medicine | Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans[ScienceDirect: S0891584913006345] |
| Frontiers in Oncology | Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation[Frontiersin:Source] |
| Tissue Engineering Part A | Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells[Pubmed:24813640] |
| Immunobiology. | The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered[Pubmed:24947894] |
| Apoptosis | Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes[Pubmed:25577277] |
| Front Oncol | Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation[PubMed: 26029659] |
| Acta Universitatis Tamperensis | Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties[Handle: 10024] |
| PLoS One | Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase[pubmed:27695127] |
| Journal of Biomedical Nanotechnology | Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility[Pubmed:29883557] |
| Biochemistry | Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells[] |
| American Journal of Pathology | Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice[Pubmed: 30980800] |
| international immunopharmacology | Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression[Pubmed: 31176083] |
| Stem Cell Reports | Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4[] |
| International Journal of?Tryptophan?Research | Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes??-[33343199] |
| Stem Cell Reports | Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4[] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?…[34802039] |