吻素1(KISS1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Kisspeptin 1 (KISS1)
Metastin; KiSS-1 Metastasis-Suppressor
- 編號KSC559Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍31.2-2000pg/mL
- 可適靈敏度13.1pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對吻素1(KISS1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC559Hu01 | 吻素1(KISS1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPC559Hu21 | 吻素1(KISS1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAC559Hu01 | 吻素1(KISS1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC559Hu08 | 吻素1(KISS1)多克隆抗體 | WB; IHC; ICC; IP. |
| MAC559Hu21 | 吻素1(KISS1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC559Hu22 | 吻素1(KISS1)單克隆抗體 | WB; IHC |
| SEC559Hu | 吻素1(KISS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEC559Hu | 吻素1(KISS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC559Hu | 吻素1(KISS1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC559Hu01 | 吻素1(KISS1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Mol Endocrinol. | LIM-homeodomain transcription factor Isl-1 mediates kisspeptin's effect on insulin secretion in mice.[Pubmed:24956377] |
| 臨床兒科雜志 | 雙酚A 與女童性早熟的關(guān)系[Fileup: Pdf] |
| J Pediatr Endocrinol Metab | The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarche[PubMed: 26565542] |
| Archives of Physiology and Biochemistry | Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese women[Pubmed:27011110] |
| International Journal of Endocrinology | The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages[pubmed:27990162] |
| Journal of?Clinical?Research?in Pediatric Endocrinology | Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche.[pubmed:28008860] |
| BioMed Research International | Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China[] |
| OBESITY?SURGERY | Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive Hormones[Pubmed: 30806913] |
| International Journal of Endocrinology | Serum Kisspeptin and Its Relation to Metabolic Parameters and Glucose Metabolism in Prepubertal and Pubertal Obese Children[Pubmed: 33224197] |
| journal of reproduction and fertility | KISS1R Signaling Modulates Gonadotropin Sensitivity in Mouse Leydig Cell[Pubmed: 33112765] |
| Beyond GnRH, LH and FSH: the role of kisspeptin on hypothalalmic-3 pituitary gonadal (HPG) axis pathology and diagnostic consideration 4[] | |
| Medeni Med J | Kisspeptin and Hematologic Parameters as Predictive Biomarkers for First-Trimester Abortions[34239761] |
| Arch Gynecol Obstet | Comparison of the Kisspeptin levels in early onset preeclampsia and late-onset preeclampsia[Pubmed:35039882] |
| J Pediatr Endocrinol Metab | Serum kisspeptin, leptin, neuropeptide Y, and neurokinin B levels in adolescents with polycystic ovary syndrome[Pubmed:35170267] |
| Genes | Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis[Pubmed:35205340] |