賴氨酰氧化酶(LOX)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Lysyl Oxidase (LOX)
Protein-Lysine 6-Oxidase
- 編號(hào)KSC580Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.061ng/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)賴氨酰氧化酶(LOX)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APC580Hu01 | 賴氨酰氧化酶(LOX)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC580Hu01 | 賴氨酰氧化酶(LOX)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC580Hu02 | 賴氨酰氧化酶(LOX)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC580Hu61 | 賴氨酰氧化酶(LOX)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC580Hu02 | 賴氨酰氧化酶(LOX)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC580Hu01 | 賴氨酰氧化酶(LOX)多克隆抗體 | WB; IHC |
| LAC580Hu81 | 賴氨酰氧化酶(LOX)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAC580Hu71 | 賴氨酰氧化酶(LOX)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC580Hu22 | 賴氨酰氧化酶(LOX)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC580Hu | 賴氨酰氧化酶(LOX)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEC580Hu | 賴氨酰氧化酶(LOX)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC580Hu | 賴氨酰氧化酶(LOX)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC580Hu01 | 賴氨酰氧化酶(LOX)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| American Journal of Hematology | The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms.[PubMed: 23494965] |
| Current Eye Research | High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells[Pubmed: 22966818] |
| European Journal of Surgical Oncology | Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed: 24246612] |
| Vaccine | Copia autorizada por CDR[Ingentaconnect: Source] |
| Arthritis & Rheumatology | Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosis[Pubmed: 24574233] |
| Eur J Surg Oncol.? | Evaluation of serum lysyl oxidase as a blood test for colorectal cancer[Pubmed:Pmc4237001] |
| BMC Cancer. | Ionizing radiation induces tumor cell lysyl oxidase secretion[Pubmed:Pmc4223762] |
| DNA Cell Biol. | Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical Cancer[Pubmed:24945327] |
| scandinavian journal of immunology | Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic Sclerosis[PubMed: 26095613] |
| Curr Eye Res | Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-? Levels and LOXL1 Genotype in Pseudoexfoliation[Pubmed:27116380] |
| journal of bone and mineral metabolism | LOX Fails to Substitute for RANKL in Osteoclastogenesis.[pubmed:27606829] |
| 38 | Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases.[pubmed:28445728] |
| Molecular Medicine Reports | Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen?induced arthritis[pubmed:28901438] |
| Revista Espa?ola de Cardiología?(English?Edition) | A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection Fraction[Pubmed:29807761] |
| Molecular?Medicine?Reports | Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitro[Pubmed:29393489] |
| Thyroid | Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressiveness[Pubmed: 30398411] |
| Am J Physiol Lung Cell Mol Physiol | Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis.[Pubmed: 33026236] |
| J Am Heart Assoc | Collagen Cross?\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy[33356379] |