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基質(zhì)金屬蛋白酶10(MMP10)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10)

STMY2; SL2; Stromelysin 2; Transin-2

  • 基質(zhì)金屬蛋白酶10(MMP10)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 基質(zhì)金屬蛋白酶10(MMP10)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)基質(zhì)金屬蛋白酶10(MMP10)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
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參考文獻(xiàn)

雜志參考文獻(xiàn)
Journal of Cellular and Molecular MedicineType-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling[Wiley: source]
PLoS One.Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential[PubMed: PMC3335833]
PLoS ONEA Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source]
Thromb Haemost.Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655]
Brain, Behavior, and ImmunityPlasma inflammatory biomarkers for Huntington’s disease patients and mouse model [Pubmed:25266150]
Biomedical MaterialsIn vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study[Pubmed:25634132]
Brain, Behavior, and ImmunityPlasma inflammatory biomarkers for Huntington’s disease patients and mouse model[PubMed: 25266150]