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頭蛋白(NOG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Noggin (NOG)

SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)

  • 頭蛋白(NOG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 頭蛋白(NOG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)頭蛋白(NOG)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APC130Hu01頭蛋白(NOG)活性蛋白Cell?culture;?Activity?Assays.
RPC130Hu01頭蛋白(NOG)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPC130Hu02頭蛋白(NOG)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPC130Hu61頭蛋白(NOG)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC130Hu01頭蛋白(NOG)多克隆抗體WB; IHC; ICC; IP.
PAC130Hu02頭蛋白(NOG)多克隆抗體WB; IHC; ICC; IP.
LAC130Hu71頭蛋白(NOG)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
LAC130Hu81頭蛋白(NOG)多克隆抗體(異硫氰酸熒光素標(biāo)記)WB; IHC; ICC; IF.
MAC130Hu21頭蛋白(NOG)單克隆抗體WB; IHC; ICC; IP.
MAC130Hu22頭蛋白(NOG)單克隆抗體WB; IHC
MAC130Hu24頭蛋白(NOG)單克隆抗體WB,IHC
MAC130Hu26頭蛋白(NOG)單克隆抗體WB,IHC
FAC130Hu02抗頭蛋白(NOG)單克隆抗體FCM
LAC130Hu76頭蛋白(NOG)單克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
SEC130Hu頭蛋白(NOG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC130Hu頭蛋白(NOG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
PSC130Hu01頭蛋白(NOG)抗體對(duì)ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSC130Hu01頭蛋白(NOG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
Arthritis RheumatolImbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis[PubMed: 26413886]
Arthritis Rheumatol.?Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis[Pubmed:26413886]
Clin Exp Pharmacol Physiol.Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways.[Pubmed:26385023]
European cells & materialsThe BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.[pubmed:28266688]
Arthritis?&?Rheumatologymbalance?Between?Bone?Morphogenetic?Protein?2?and?Noggin?Induces?Abnormal?OsteogenicDifferentiation?of?Mesenchymal?Stem Cells in Ankylosing Spondylitis.[pubmed:26413886]
Biotechnology and?Applied?BiochemistryThe BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells.[pubmed:28981962]
HeliyonEfficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis[pubmed:29167826]
Journal of Cellular BiochemistryLow‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular?…[Pubmed: 31006911]
Aging MaleEffect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment[Pubmed: 32160825]
J Reconstr MicrosurgEffect of Simvastatin Use in Free Tissue Transfer: An Experimental Study in a Rat Epigastric Free Flap Model[Pubmed: 31995818]
Biochem Biophys RepSlow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP[Pubmed:35005256]