骨橋素(OPN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- 編號KSA899Si01
- 物種Rhesus monkey (Simian，恒河猴)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.070ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 8748¥ 14580¥ 26244¥ 51030¥ 87480
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對骨橋素(OPN)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rhesus monkey (Simian，恒河猴) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA899Si01 | 骨橋素(OPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA899Si01 | 骨橋素(OPN)多克隆抗體 | WB,IHC |
| SEA899Si | 骨橋素(OPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA899Si | 骨橋素(OPN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA899Si01 | 骨橋素(OPN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| International Journal of Pharmaceutics | Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester)[ScienceDirect: S0378517312003742] |
| European Cells and matreials | The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering[Ecmjournal: Source] |
| Journal of Equine Veterinary Science | Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse[ScienceDirect: Source] |
| Basic & Clinical Pharmacology & Toxicology | Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions[Wiley:Source] |
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| Journal of Cardiovascular Electrophysiology | Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.[pubmed:28127817] |
| American Journal of Translational Research | Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes.[pubmed:28337262] |
| Diabetes Care | Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans[pubmed:29212824] |
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| Journal of?Alzheimers?Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods[Pubmed:30040720] |
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| Journal of Clinical Medicine | Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and …[Pubmed: 32182704] |
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| [ENGINEERING OF BIOMATERIALS | Osteoconductive potential of PLGA/bioglass composite biomaterials[] |
| Biomolecules | Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44[34070790] |
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