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血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Platelet Derived Growth Factor Subunit A (PDGFA)

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

  • 血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產品包裝(模擬)
  • 血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對血小板衍生生長因子A(PDGFA)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產品

編號適用物種:Homo sapiens (Human,人)應用(僅供研究使用,不用于臨床診斷!)
RPA528Hu01血小板衍生生長因子A(PDGFA)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA528Hu01血小板衍生生長因子A(PDGFA)活性蛋白Cell?culture;?Activity?Assays.
LAA528Hu71血小板衍生生長因子A(PDGFA)多克隆抗體(生物素標記)WB; IHC; ICC.
LAA528Hu81血小板衍生生長因子A(PDGFA)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
MAA528Hu22血小板衍生生長因子A(PDGFA)單克隆抗體WB; IHC; ICC; IP.
MAA528Hu24血小板衍生生長因子A(PDGFA)單克隆抗體WB; IHC; ICC; IP.
MAA528Hu21血小板衍生生長因子A(PDGFA)單克隆抗體WB; IHC; ICC; IP.
MAA528Hu23血小板衍生生長因子A(PDGFA)單克隆抗體ICC/IF
SEA528Hu血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA528Hu血小板衍生生長因子A(PDGFA)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA528Hu01血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志參考文獻
Canadian Journal of Physiology and PharmacologyThe effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats[pubmed:28177680]
International ImmunopharmacologyShikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway[pubmed:28521243]
Open Access Macedonian Journal of Medical SciencesLow Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells[]
Prostaglandins & other Lipid MediatorsLipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway[Pubmed: 31698141]
Ann Agric Environ MedPDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis[Pubmed: 32208584]
Tissue Eng Regen MedEffect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method[34339025]
Biomed Research InternationalThermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form[Pubmed:35586817]