P物質(zhì)(SP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Substance P (SP)
- 編號KSA393Hu11
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度5.24pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對P物質(zhì)(SP)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| SPA393Hu01 | P物質(zhì)(SP)合成多肽 | Immunogen; Blocking Peptide. |
| PAA393Hu01 | P物質(zhì)(SP)多克隆抗體 | WB; IHC; ICC; IP. |
| CEA393Hu | P物質(zhì)(SP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA393Hu | P物質(zhì)(SP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA393Hu | P物質(zhì)(SP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA393Hu11 | P物質(zhì)(SP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Veterinary Internal Medicine | A Potential Role for Substance P and Interleukin-6 in the Cerebrospinal Fluid of Cavalier King Charles Spaniels with Neuropathic Pain[Pubmed: 23659719] |
| Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation[PubMed: 25836921] |
| Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation[10.1007/s13765-017-0327-3] |
| CNS?Neuroscience & Therapeutics? | Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulation[Pubmed:29900692] |
| Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement[Pubmed: 30562932] |
| Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma[Pubmed: 31542455] |
| Chinese Medicine | Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model[Pubmed: 33014123] |
| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy[] |
| Int Urol Nephrol | Evaluation of the causes affecting the development of pruritus in patients with peritoneal dialysis[34213714] |
| J Chem Neuroanat | Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice[34626771] |