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多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)多配體蛋白聚糖1(SDC1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB966Mu02多配體蛋白聚糖1(SDC1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB966Mu01多配體蛋白聚糖1(SDC1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPB966Mu62多配體蛋白聚糖1(SDC1)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB966Mu01多配體蛋白聚糖1(SDC1)多克隆抗體WB; IHC; ICC; IP.
PAB966Mu02多配體蛋白聚糖1(SDC1)多克隆抗體WB; IHC; ICC; IP.
SEB966Mu多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB966Mu多配體蛋白聚糖1(SDC1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSB966Mu01多配體蛋白聚糖1(SDC1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
Molecular and Cellular Biochemistry Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction[SpringerLink: a201w3l18k257462]
Inflammation Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats[Springer: Source]
Atherosclerosis.Damage of the endothelial glycocalyx in chronic kidney disease[Pubmed:24727235]
Journal of Trauma and Acute Care SurgeryEffect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury[Pubmed:25058256]
ShockImpairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance[Pubmed:25692257]
Mediators InflammDexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding[PubMed: 26199464]
TransplantationPlatelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury.[PubMed: 26371596]
Clinical and Experimental NephrologyDual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury[pubmed:27405618]
Biomarkers in MedicineEndothelial glycocalyx layer shedding following lung resection[pubmed:27643669]
Danish National RESEARCH DATABASEResuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats[10.1089]
Journal of NeurotraumaResuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat.[doi/10.1089/neu.2016.4574]
British Journal of AnaesthesiaVolume kinetics of Ringer's lactate solution in acute inflammatory disease[10.1016:j.bja.2018.04.023]
Arteriosclerosis, Thrombosis, and?Vascular BiologyMPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge[Pubmed:29903730]
ShockPlasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis[Pubmed:28591008]
ShockExperimental models of endotheliopathy: impact of shock severity[Pubmed:28697004]
Journal of?Trauma?and?Acute?Care?SurgeryEffects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat[Pubmed:28930945]
The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions[173015_06.pdf]
Journal of Surgical ResearchPlasma ameliorates endothelial dysfunction in burn injury[Doi: 10.1016/j.jss.2018.08.027]
Journal of AnesthesiaDexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model[Pubmed: 30374889]
AnaesthesiaPostoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass[Pubmed: 30687934]
Journal of Cardiothoracic and Vascular AnesthesialMicrovascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit[Pubmed: 31787433]
Hepatobiliary & Pancreatic Diseases InternationalIncreased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP[Pubmed: 31919038]
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study[]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESNewly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis[33375342]
Microvasc ResEffect of liraglutide on microcirculation in rat model with absolute insulin deficiency[34119534]
J Gerontol A Biol Sci Med SciThe effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo?…[34423832]
ShockInhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke[34524269]
Exp AnimDexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model[Pubmed:35110424]
Journal of Emergency MedicineResuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase[]