促甲狀腺素釋放激素(TRH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Thyrotropin Releasing Hormone (TRH)
TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
- 編號(hào)KSA839Mu11
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma and other biological fluids.
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍6.17-500pg/mL
- 可適靈敏度2.39pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)促甲狀腺素釋放激素(TRH)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA839Mu01 | 促甲狀腺素釋放激素(TRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA839Mu02 | 促甲狀腺素釋放激素(TRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA839Mu21 | 促甲狀腺素釋放激素(TRH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA839Mu31 | 促甲狀腺素釋放激素(TRH)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA839Mu08 | 促甲狀腺素釋放激素(TRH)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA839Mu01 | 促甲狀腺素釋放激素(TRH)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA839Mu02 | 促甲狀腺素釋放激素(TRH)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA839Mu81 | 促甲狀腺素釋放激素(TRH)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| CEA839Mu | 促甲狀腺素釋放激素(TRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA839Mu | 促甲狀腺素釋放激素(TRH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA839Mu11 | 促甲狀腺素釋放激素(TRH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Plos one | Thyrotropin-Releasing Hormone (TRH) Promotes Wound Re-Epithelialisation in Frog and Human Skin[PubMed: PMC3759422] |
| Analyst | Ultrasensitive detection of thyrotropin-releasing hormone based on azo coupling and surface-enhanced resonance Raman spectroscopy[Pubmed:27338554] |
| journal of neuroendocrinology | Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder.[pubmed:28199771] |
| Journal of Neuroendocrinology | Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder[10.1111/jne.12461] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice[Pubmed:29403355] |
| Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice[Pubmed: 30404008] |