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玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Vitronectin (VTN)

V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

  • 玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對玻連蛋白(VTN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB041Hu02玻連蛋白(VTN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB041Hu03玻連蛋白(VTN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB041Hu01玻連蛋白(VTN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB041Hu02玻連蛋白(VTN)多克隆抗體WB; IHC; ICC; IP.
PAB041Hu01玻連蛋白(VTN)多克隆抗體WB; IHC; ICC; IP.
PAB041Hu03玻連蛋白(VTN)多克隆抗體WB; IHC; ICC; IP.
FAB041Hu01抗玻連蛋白(VTN)多克隆抗體Flow cytometry.
FAB041Hu03抗玻連蛋白(VTN)多克隆抗體Flow cytometry.
LAB041Hu71玻連蛋白(VTN)多克隆抗體(生物素標記)WB; IHC; ICC.
MAB041Hu22玻連蛋白(VTN)單克隆抗體WB; IHC; ICC; IP.
SEB041Hu玻連蛋白(VTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB041Hu玻連蛋白(VTN)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
PSB041Hu01玻連蛋白(VTN)抗體對ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB041Hu01玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志參考文獻
Biomedical Engineering and InformaticsApplication of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer[IEEE: 6098563]
PLoS ONEIdentification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance[PubMed: PMC3754973]
Liver InternationalIdentification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE[Pubmed: 23944848]
Analytical BiochemistryA multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots[Pubmed:29154788]
Journal of Materials Chemistry BSerum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs[Doi: 10.1039/C8TB02283F?]
JOURNAL OF NEPHROLOGYUrinary vitronectin identifies patients with high levels of fibrosis in kidney grafts[33275196]