無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
- 編號(hào)KSP549Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.059ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPP549Hu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP549Hu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)多克隆抗體 | WB; IHC; ICC; IP. |
| MAP549Hu21 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)單克隆抗體 | WB; IHC; ICC; IP. |
| SEP549Hu | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMP549Hu | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSP549Hu01 | 無翅型MMTV整合位點(diǎn)家族成員5A(WNT5A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Liver International | Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis[Pubmed:25488180] |
| Journal of Physiology and Biochemistry | Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells[PubMed: 26547443] |
| Liver | Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis[PubMed: 25488180] |
| Transl Res | Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures[PubMed: 26163991] |
| 不確定雜志名 | Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an …[servlets:MCRFileNodeServlet] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?…[Pubmed:29674475] |
| Stem?Cells | Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells[Pubmed:29377497] |
| Science Translational Medicine | Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases[Pubmed: 31534019] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans[Pubmed: 30137542] |
| Cancer Research | Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis[Pubmed: 31932454] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease[Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …[Pubmed: 32491086] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties[34823885] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study[Pubmed:34990470] |
| J Clin Periodontol | Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study[Pubmed:35569034] |