白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Albumin (ALB)
Serum albumin
- 編號SEB028Si
- 物種Rhesus monkey (Simian，恒河猴)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ug/mL
- 靈敏度最小可檢測劑量小于等于0.067ug/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測白蛋白(ALB)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 85-98 | 94 |
| heparin plasma(n=5) | 88-97 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白蛋白(ALB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 80-92% | 92-104% | 79-105% |
| EDTA plasma(n=5) | 91-105% | 92-101% | 79-94% | 98-105% |
| heparin plasma(n=5) | 82-89% | 81-99% | 80-101% | 78-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將白蛋白(ALB)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的白蛋白(ALB)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白蛋白(ALB)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的白蛋白(ALB)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rhesus monkey (Simian，恒河猴) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPB028Si01 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB028Si01 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB028Si71 | 白蛋白(ALB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB028Si | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation[PubMed: 22701728] |
| Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups[Onlinelibrary: liv.12219] |
| Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice[Pubmed: 24273183] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients[Pubmed:25590278] |
| Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice[PubMed: 26346164] |
| PLoS?One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study[Pubmed:26934615] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering[art00007] |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy.[pubmed:29246788] |
| Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury[Pubmed:30014231] |
| Journal of Developmental Origins of?Health?and?Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring[Pubmed:29582727] |
| Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study[Pubmed:29384875] |
| Stem?Cell?Research?&?Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure[Pubmed: 30463600] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection[Pubmed: 30963688] |
| biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization[Pubmed: 31146107] |
| biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice[Pubmed: 31256934] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation[Pubmed: 31447572] |
| Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity[Pubmed: 31580898] |
| BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia[Pubmed: 31655595] |
| International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway[Pubmed: 31918060] |
| HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway[Pubmed: 32908237] |
| Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats[Pubmed: 32399849] |
| Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats[] | |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats[] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate[] |
| Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling[33721834] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle[] |
| Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway[34807349] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity[34853313] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions[34914377] |
| Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice[Pubmed:35708145] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy[] |
