血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Angiotensin 1-7 (Ang1-7)
- 編號CES085Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍12.35-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.69pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3108¥ 4440¥ 19980¥ 37740¥ 310800
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測血管緊張素1-7(Ang1-7)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血管緊張素1-7(Ang1-7)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-103 | 97 |
| EDTA plasma(n=5) | 80-89 | 79 |
| heparin plasma(n=5) | 86-94 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管緊張素1-7(Ang1-7)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素1-7(Ang1-7)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 79-98% | 95-105% | 78-105% |
| EDTA plasma(n=5) | 95-103% | 96-104% | 79-105% | 96-104% |
| heparin plasma(n=5) | 83-96% | 92-104% | 92-101% | 89-97% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將血管緊張素1-7(Ang1-7)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質(zhì)含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPS085Hu21 | 血管緊張素1-7(Ang1-7)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAS085Hu01 | 血管緊張素1-7(Ang1-7)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAS085Hu71 | 血管緊張素1-7(Ang1-7)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| CES085Hu | 血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCS085Hu | 血管緊張素1-7(Ang1-7)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMS085Hu | 血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSS085Hu11 | 血管緊張素1-7(Ang1-7)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| J Stroke Cerebrovasc Dis | Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan[PubMed: 25891757] |
| Cellular Physiology and Biochemistry | Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats[pubmed:28848201] |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression[pubmed:29066981] |
| Molecular Medicine Reports | The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction.[pubmed:28656296] |
| Journal of Clinical Pharmacy and Therapeutics | Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites.[pubmed:28895159] |
| Molecular Medicine Reports | Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats[pubmed:28944898] |
| Open Journal of Endocrine and Metabolic Diseases Paper Submission | A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats[2-1980272_83198.htm] |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system[Pubmed:29752040] |
| Innate?Immunity? | Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis[Pubmed:29969931] |
| Evidence-based?Complementary and Alternative?Medicine? | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects[Pubmed:29977313] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | The impact of ovulation inducing drugs on carcinogenesis[] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia[] |
| Journals of Gerontology Series A-Biological Sciences and Medical Sciences | Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study.[Pubmed: 31586210] |
| journal of the european academy of dermatology and venereology | Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis[Pubmed: 31746507] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181] |
| Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?…[] | |
| Amino Acids | Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats[33398523] |
| Autoimmunity | The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis[33910447] |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice[] |
| Peptides | Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide[34298021] |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice[34548056] |
| J Cardiovasc Pharmacol | Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy[34232225] |
