腦源性神經(jīng)營養(yǎng)因子(BDNF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)
Neurotrophin; Abrineurin
- 編號SEA011Gu
- 物種Cavia (Guinea pig ，豚鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.057ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測腦源性神經(jīng)營養(yǎng)因子(BDNF)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的腦源性神經(jīng)營養(yǎng)因子(BDNF)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-102 | 91 |
| EDTA plasma(n=5) | 90-97 | 93 |
| heparin plasma(n=5) | 92-99 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腦源性神經(jīng)營養(yǎng)因子(BDNF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腦源性神經(jīng)營養(yǎng)因子(BDNF)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-99% | 88-102% | 80-105% | 88-102% |
| EDTA plasma(n=5) | 78-88% | 96-103% | 93-101% | 88-95% |
| heparin plasma(n=5) | 78-89% | 82-93% | 88-97% | 78-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將腦源性神經(jīng)營養(yǎng)因子(BDNF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的腦源性神經(jīng)營養(yǎng)因子(BDNF)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腦源性神經(jīng)營養(yǎng)因子(BDNF)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的腦源性神經(jīng)營養(yǎng)因子(BDNF)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Cavia (Guinea pig ，豚鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA011Gu01 | 腦源性神經(jīng)營養(yǎng)因子(BDNF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA011Gu01 | 腦源性神經(jīng)營養(yǎng)因子(BDNF)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA011Gu21 | 腦源性神經(jīng)營養(yǎng)因子(BDNF)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA011Gu | 腦源性神經(jīng)營養(yǎng)因子(BDNF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA011Gu | 腦源性神經(jīng)營養(yǎng)因子(BDNF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Toxicological Sciences | Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat Brains[PubMed: 21436126] |
| Journal of the Neurological Sciences | Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord[ScienceDirect: S0022510X12001219] |
| International Conference on Bioscience | Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage[Psrcentre: 212059] |
| Neuroscience Letters | Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice[ScienceDirect: S0304394010015788] |
| BioMed Research International | Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.[NCBI: PMC3925622] |
| Behavioural Brain Research | The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor[Pubmed:25446810] |
| Neurochemical Research | Heme?Oxygenase-1?Protects?Neurons?from?Ischemic?Damage?by?UpreCavia (Guinea pig )lating?Expression?of?Cu,Zn-SuperoxideDismutase,?Catalase, and?Brain-Derived?Neurotrophic?Factor?in the?Rabbit?Spinal?Cord.[Pubmed:26559686] |
| Exp Ther Med. | Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats[pmc:PMC4812310] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells[Pubmed:27323064] |
| The International Journal af Applied Research in Veterinary Medicine | Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study [articles:Vol14Iss3] |
| J Chem Neuroanat. | Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.[pubmed:27914953] |
| Int Immunopharmacol. | PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice.[pubmed:27940378] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities[pubmed:28081639] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …[articles:journal-of-alzheimers-disease] |
| Bipolar Disorders | Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic …[bdi.12449] |
| Elsevier | Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls[science:article] |
| journal of?allergy?and?clinical?immunology | Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis[pubmed:28412394] |
| American Journal of Translational Research | A new comorbidity model and the common pathological mechanisms of migraine and epilepsy[pubmed:28559979] |
| European Journal of Pharmacology | Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal.[pubmed:29042206] |
| Brain,?Behavior, and?Immunity | An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling[Pubmed:29627530] |
| Stem?Cell?Research?&?Therapy | Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model[Pubmed:29335016] |
| Pharmacological?Research? | N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype[Pubmed:29203442] |
| Experimental Neurology | Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia[Pubmed:30012511] |
| Nutrients | Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model.[Pubmed:29890692] |
| Neurotoxicity Research | Delta-9-Tetrahydrocannabinol (?-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats[Pubmed:28933048] |
| Neurotoxicology | Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats[Pubmed:29522778] |
| Neuroscience Letters | Long term Westernized diet leads to region-specific changes in brain signaling mechanisms[Pubmed:29655945] |
| Applied?Physiology Nutrition and Metabolism | Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists[Pubmed: 30286300] |
| Heliyon | Establishment and evaluation of a novel mouse model of peri/postmenopausal depression[Doi: 10.1016/j.heliyon.2019.e01195] |
| BioMed Research International | Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling[] |
| International Journal of Immunopathology and Pharmacology | Effect of voluntary running on expression of myokines in brains of rats with depression[Pubmed: 30834799] |
| American Journal of Emergency?Medicine | Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute stroke[Pubmed: 30826210] |
| Neurochemistry International | Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in mice[Pubmed: 31077758] |
| REVISTA DA ASSOCIACAO MEDICA BRASILEIRA | Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression[Pubmed: 30994834] |
| Journal of Alzheimer's Disease | Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model Mice[Pubmed: 31561359] |
| Biological Trace Element Research | Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling[Pubmed: 31797207] |
| Psychopharmacology | Chronic harmine treatment has a delayed effect on mobility in control and socially defeated rats[Pubmed: 32088835] |
| Experimental and Therapeutic Medicine | Involvement of neurotrophic signaling in doxorubicin?induced cardiotoxicity[Pubmed: 32010279] |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness[Pubmed: 31896448] |
| Journal of Clinical Neuroscience | CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic children[Pubmed: 32327377] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and?…[Pubmed: 32918393] |
| Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder[Pubmed: 32791084] |
| COLLOIDS AND SURFACES B-BIOINTERFACES | Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease[Pubmed: 32777662] |
| Neurotoxicology | Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice[Pubmed: 32522471] |
| Journal of Veterinary Behavior | Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety[] |
| Food & Function | Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial?…[] |
| Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine[] | |
| bioinformatics and biomedical engineering | Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke[] |
| Overektomize di?i s??anlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile n?rodavran??sal de?i?iklikler üzerine anjiyotensin II ?nhibisyonunun etkisi[] | |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??-[33389158] |
| BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways[33589896] |
| Neuropsychiatr Dis Treat | Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567?…[34239303] |
| Einstein (Sao Paulo) | Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy[34586159] |
| Metab Brain Dis | Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men[34762211] |
| Transl Psychiatry | Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression[34824208] |
| Iranian Journal of Basic Medical Sciences | Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons[34712429] |
| J Sex Med | Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid?…[34384732] |
| Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway[34296452] |
| J Endocrinol Invest | Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader?…[Pubmed:35098494] |
| Clinical Rheumatology | Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by?…[Pubmed:35344113] |
| Sci Rep | Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients[Pubmed:35508633] |
| Pharmaceutics | Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord?…[Pubmed:35214053] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?…[Pubmed:35585377] |
| Journal of Complementary Medicine Research | Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder[] |
| AFRICAN JOURNAL OF BIOCHEMISTRY RESEARCH | Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress[] |
