促甲狀腺素(TSH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Thyroid Stimulating Hormone (TSH)
Thyrotropin
- 編號CEA463Ca
- 物種Canis familiaris; Canine (Dog，犬)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍24-15,000pg/mL
- 靈敏度最小可檢測劑量小于等于11pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3024¥ 4320¥ 19440¥ 36720¥ 302400
- 欲了解實(shí)際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測促甲狀腺素(TSH)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的促甲狀腺素(TSH)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-94 | 87 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 79-103 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的促甲狀腺素(TSH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中促甲狀腺素(TSH)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-104% | 94-101% | 87-94% | 95-103% |
| EDTA plasma(n=5) | 78-93% | 80-104% | 95-103% | 89-97% |
| heparin plasma(n=5) | 85-94% | 98-105% | 82-91% | 97-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將促甲狀腺素(TSH)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA463Ca | 促甲狀腺素(TSH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA463Ca | 促甲狀腺素(TSH)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA463Ca | 促甲狀腺素(TSH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA463Ca11 | 促甲狀腺素(TSH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Blood | Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID[Pubmed: 22184407] |
| Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies[Lifesciencesite: Source] |
| Thyroid | Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function[Pubmed: 23819433] |
| Journal of Functional Foods | Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats[Sciencedirect:S1756464615000894] |
| J Endocrinol. | Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis[Pubmed:24928937] |
| journal of lipid research | Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver[PubMed: 25713102] |
| Int J Clin Exp Med | Lead exposure causes thyroid abnormalities in diabetic rats[PubMed: 26221254] |
| Jubilant Drax Image | Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal Brain[Pubmed:27231981] |
| PLoS One. | The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats[Pubmed:27043135] |
| journal of bone and mineral research | Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis.[pubmed:27589370] |
| Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring[pubmed:27803384] |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.[pubmed:28322267] |
| Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders[handle:123456789] |
| International Journal of Peptide Research and Therapeutics | Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins[article:10.1007] |
| Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats[10.4314/tjpr.v16i5.9] |
| 30 | The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis.[pubmed:28546511] |
| NITRIC OXIDE-BIOLOGY AND CHEMISTRY | Voluntary exercise training restores anticontractile effect of NO in coronary arteries of adult rats with antenatal/early postnatal hypothyroidism[Pubmed:29307633] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population[Pubmed:29525625] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice[Pubmed:29403355] |
| Neuroscience Letter | Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats[Pubmed:29574218] |
| Journal of?Neuroinflammation | Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice[Pubmed: 30373627] |
| Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice[Pubmed: 30404008] |
| Journal of Diabetes Research | The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice[] |
| Endocrine | Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity[Pubmed: 31056723] |
| Oxidative Medicine and Cellular Longevity | Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of?…[] |
| Reproductive Biology and Endocrinology | Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice[Pubmed: 31729993] |
| Gli | Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice[Pubmed: 32173924] |
| Clinical Immunology | The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice[Pubmed: 31982645] |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis[Pubmed: 33177714] |
| Thyroid | Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation[Pubmed: 32924834] |
| Thyroid | Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid mice[Pubmed: 32907517] |
| Int Immunopharmacol | Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy[Pubmed: 32442899] |
| Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposi??o materna ao triclosan causa?…[] |
| Comparaison des marqueurs hormonaux et histomorphométriques chez la souris un modèle d'hypothyro?die après 28 jours de traitement au PTU par voie alimentaire.[] | |
| Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine?\treated rat dams during pregnancy and lactation[33433934] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart[33375430] |
| Biomedicines | Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 131[33572949] |
| Mol Neurobiol | Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75 NTR Signal Pathway[33966253] |
| Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism[34092241] |
| Pharmaceutics | Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays[] |
| BRITISH JOURNAL OF NUTRITION | Effects of iodine excess on serum thyrotropin-releasing hormone levels and type 2 deiodinase in the hypothalamus of Wistar rats[34250878] |
| Gene Therapy | Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector[34599290] |
| NatCommun | Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis[34876572] |
| Int J Mol Sci | The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism[Pubmed:35163456] |
| Molecular Biology Reports | Effects of meteorin-like hormone on endocrine function of hypothalamo-hypophysial system and peripheral uncoupling proteins in rats[Pubmed:35332411] |
