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18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

  • 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)實(shí)驗(yàn)結(jié)果圖
  • SEC419Hu.jpg標(biāo)準(zhǔn)曲線圖
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測18kDa陽離子抗菌肽(CAMP),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的18kDa陽離子抗菌肽(CAMP)(加標(biāo)樣品),重復(fù)測定并計(jì)算其均值,回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)89-10398
EDTA plasma(n=5)95-10399
heparin plasma(n=5)79-9288

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定,每個(gè)樣本使用同一試劑盒重復(fù)測定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的18kDa陽離子抗菌肽(CAMP),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中18kDa陽離子抗菌肽(CAMP)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)78-92%88-95%96-103%91-101%
EDTA plasma(n=5)80-103%92-105%89-105%80-93%
heparin plasma(n=5)80-102%87-96%95-104%99-105%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時(shí);
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將18kDa陽離子抗菌肽(CAMP)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的18kDa陽離子抗菌肽(CAMP)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的18kDa陽離子抗菌肽(CAMP)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的18kDa陽離子抗菌肽(CAMP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值),計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPC419Hu0118kDa陽離子抗菌肽(CAMP)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CPC419Hu2118kDa陽離子抗菌肽(CAMP)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
PAC419Hu0218kDa陽離子抗菌肽(CAMP)多克隆抗體WB; IHC; ICC; IP.
PAC419Hu0118kDa陽離子抗菌肽(CAMP)多克隆抗體WB; IHC; ICC; IP.
PAC419Hu0818kDa陽離子抗菌肽(CAMP)多克隆抗體WB; IHC; ICC; IP.
LAC419Hu7118kDa陽離子抗菌肽(CAMP)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
MAC419Hu2218kDa陽離子抗菌肽(CAMP)單克隆抗體WB; IHC; ICC; IP.
MAC419Hu2818kDa陽離子抗菌肽(CAMP)單克隆抗體WB
MAC419Hu2118kDa陽離子抗菌肽(CAMP)單克隆抗體WB
SEC419Hu18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
CEC419Hu18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC419Hu18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSC419Hu1118kDa陽離子抗菌肽(CAMP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source]
Clin Infect Dis.Host immune response to tuberculous meningitis[Pubmed:25301213]
VaccineOn birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment[Pubmed:25444792]
UrologyModulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells [Pubmed:25681250]
UrologyModulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells[PubMed: 25681250]
Sarcoidosis Vasc Diffuse Lung DisCathelicidin as a link between sarcoidosis and tuberculosis[PubMed: 26422567]
Allergy and Asthma ProceedingsThe association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children[PubMed: 26108071]
J Clin Microbiol.Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.[Pubmed:27008883]
experimental dermatologyLight-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.[pubmed:27315464]
PLOS?ONEReduced?Expression?of the?Extracellular?Calcium-Sensing?Receptor?(CaSR) Is?Associated?withActivation?of the?Renin-Angiotensin?System?(RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.[pubmed:27391973]
Front Pharmacol.?Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.[pubmed:28261091]
Allergologia et immunopathologiaThe relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin[pubmed:27955890]
Chinese Journal of Osteoporosis and Bone Mineral ResearchEffects of cold exposure on the bone marrow adipose tissue[DOI: 10.3969/j.issn.1674-2591.2017.05.008]
Scientific ReportsMeasurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique[Pubmed:29374283]
Experimental ParasitologyAntimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin[Pubmed: 30904694]
TheranosticsMyeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling[Pubmed: 31149039]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESShort-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37[Pubmed: 32050475]
MICROB PATHOGENESISHypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy[Pubmed: 32645421]
International Journal of NanomedicineEffect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide[Pubmed: 32606669]
博士論文Rela??es causais entre a express?o dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com?…[]
PLoS OneBeneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies[33999928]
New MedAnalysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between??-[]
Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea[]
Int J Mol SciCompartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection[Pubmed:35328414]
Cell RepLL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity[Pubmed:35649354]