干擾素β(IFNb)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Interferon Beta (IFNb)
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
- 編號SEA222Ga
- 物種Chicken (Gallus，雞)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測干擾素β(IFNb)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的干擾素β(IFNb)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-103 | 99 |
| EDTA plasma(n=5) | 84-101 | 94 |
| heparin plasma(n=5) | 81-88 | 85 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的干擾素β(IFNb)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中干擾素β(IFNb)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-98% | 79-91% | 98-105% | 79-93% |
| EDTA plasma(n=5) | 89-96% | 86-99% | 87-105% | 83-101% |
| heparin plasma(n=5) | 87-101% | 96-103% | 80-93% | 90-104% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將干擾素β(IFNb)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的干擾素β(IFNb)與連接于固相載體上的抗體結合，然后加入生物素化的干擾素β(IFNb)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的干擾素β(IFNb)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA222Ga01 | 干擾素β(IFNb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA222Ga01 | 干擾素β(IFNb)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA222Ga71 | 干擾素β(IFNb)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA222Ga21 | 干擾素β(IFNb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA222Ga24 | 干擾素β(IFNb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA222Ga26 | 干擾素β(IFNb)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA222Ga76 | 干擾素β(IFNb)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| HEA222Ga | 干擾素β(IFNb)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA222Ga | 干擾素β(IFNb)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA222Ga | 干擾素β(IFNb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA222Ga01 | 干擾素β(IFNb)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA222Ga01 | 干擾素β(IFNb)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| 20 | Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway[PubMed: 21355876] |
| Journal of General Virology | Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3.[Sgm: 9182080] |
| Biochemical and Biophysical Research Communications | Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage[ScienceDirect: S0006291X09019822] |
| PLoS One | Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys[PubMed: PMC3757038] |
| PLoS Neglected Tropical Diseases | Innate Immune Response to Rift Valley Fever Virus in Goats[PubMed: PMC3335883] |
| BioMed Research International | Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages[Pubmed: 24288685] |
| Vet Microbiol. | An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages.[Pubmed: 23850441] |
| Food and Chemical Toxicology | Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice[Pubmed: 23535186] |
| Veterinary immunology and immunopathology | Experimental model of equine alveolar macrophage stimulation with TLR ligands[Pubmed: 23815824] |
| American journal of respiratory cell and molecular biology | Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages[Pubmed: 24502337] |
| FEBS Lett. | Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity[Pubmed:24530528] |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain[Pubmed:25106750] |
| PLOS Neglected Tropical Diseases | MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production[Pubmed:25122182] |
| Veterinary Research | The innate immune response of equine bronchial epithelial cells is altered by training[Pubmed:25595212] |
| molecular and celluar biology | SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity[PubMed: 25939384] |
| Arch Virol | Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus[PubMed: 25797196] |
| J | Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells[PubMed: 26258404] |
| Virus | PKR activation enhances replication of classical swine fever virus in PK-15 cells[PubMed: 25899421] |
| PLoS?One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro[Pubmed:26886455] |
| Scientific Reports | Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4[Pubmed:27090571] |
| Front Microbiol | Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo[Pubmed:27252695] |
| Canadian Journal of Physiology and Pharmacology | Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25.[pubmed:28134560] |
| International Journal of Molecular Medicine | Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function[pubmed:27176664] |
| International Journal of?Clinical?and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells[files:ijcep0036479.pdf] |
| Poultry?Science | ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages[pubmed:27486255] |
| Journal?of?Virology | Characterization?of?Simian?Immunodeficiency?Virus?Variants?Anatomically?Compartmentalized?inPlasma?and?Milk?in?Chronically?Infected?African?Green?Monkeys.[pubmed:] |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B[pubmed:28342861] |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection[pubmed:28988058] |
| EMBO?Journal | Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis[Pubmed:30065070] |
| Toxicology and?applied?pharmacology | Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice[Pubmed:30017639] |
| Archives of Virology | MAVS induces a host cell defense to inhibit CSFV infection[Pubmed:29556776] |
| Journal?of?virology | Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation[Pubmed: 30518647] |
| Veterinary Immunology and Immunopathology | Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?…[Doi: 10.1016/j.vetimm.2019.01.003] |
| Science?Signaling | HIPK2 is necessary for type I interferon–mediated antiviral immunity[Doi: 10.1126/scisignal.aau4604] |
| INTERNATIONAL MEDICAL RESEARCH | Evaluation of serum interferons in patients with age-related macular degeneration[Pubmed: 31007694] |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens[] |
| International journal of biological macromolecules | Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction[Pubmed: 31284005] |
| Journal of virology | Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation[Pubmed: 31243133] |
| The Journal of Latvian Academy of Sciences | Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia[] |
| Cancer Medicine | The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4[Pubmed: 31218844] |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1[] |
| PLOS Pathogens | Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion[Pubmed: 31539404] |
| Autophagy | Induction of autophagy and suppression of type I IFN secretion by CSFV[Pubmed: 32160078] |
| MOLECULAR IMMUNOLOGY | BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation[Pubmed: 33053462] |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection[Pubmed: 32924761] |
| Arch Virol . | Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway[Pubmed: 32532134] |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon?…[] |
| bioRxiv | Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance[] |
| Res Vet Sci | Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells[34416463] |
| Eur J Immunol | Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity[Pubmed:35419822] |
| ResearchSquare | Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I[] |
