男人女人午夜视频免费,免费人妻无码不卡中文字幕系,国产A级三级三级三级,成年无码aⅴ片在线观看

淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)

  • 淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)實(shí)驗(yàn)結(jié)果圖
  • CEA864Hu.jpg標(biāo)準(zhǔn)曲線圖
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)淀粉樣蛋白β1-40(Ab1-40),經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的淀粉樣蛋白β1-40(Ab1-40)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)84-10398
EDTA plasma(n=5)80-10186
heparin plasma(n=5)94-10197

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的淀粉樣蛋白β1-40(Ab1-40),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中淀粉樣蛋白β1-40(Ab1-40)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)91-101%78-91%89-96%80-90%
EDTA plasma(n=5)85-102%96-104%85-96%78-101%
heparin plasma(n=5)88-95%78-94%86-93%98-105%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)50µL,
    加入50µL檢測(cè)液A(臨用前配制);
    37°C溫育1小時(shí)。
3. 洗板3次;
4. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分鐘;
7. 加終止液50µL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將淀粉樣蛋白β1-40(Ab1-40)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本),待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物,然后加入HRP標(biāo)記的親和素,經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。待測(cè)標(biāo)本濃度越高,標(biāo)記抗原和抗體的結(jié)合就越受到抑制,顯色愈淺。顯色的深淺與酶量呈正相關(guān),而與樣品中待測(cè)物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA864Hu01淀粉樣蛋白β1-40(Ab1-40)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CPA864Hu12淀粉樣蛋白β1-40(Ab1-40)牛血清白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPA864Hu23淀粉樣蛋白β1-40(Ab1-40)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPA864Hu22淀粉樣蛋白β1-40(Ab1-40)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPA864Hu11淀粉樣蛋白β1-40(Ab1-40)牛血清白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPA864Hu21淀粉樣蛋白β1-40(Ab1-40)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPA864Hu31淀粉樣蛋白β1-40(Ab1-40)鑰孔血藍(lán)蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
SPA864Hu02淀粉樣蛋白β1-40(Ab1-40)合成多肽Immunogen; Blocking Peptide.
PAA864Hu08淀粉樣蛋白β1-40(Ab1-40)多克隆抗體WB
PAA864Hu01淀粉樣蛋白β1-40(Ab1-40)多克隆抗體WB; IHC
CEA864Hu淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA864Hu淀粉樣蛋白β1-40(Ab1-40)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA864Hu11淀粉樣蛋白β1-40(Ab1-40)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
PLoS OneMetabolites of Cerebellar Neurons and Hippocampal Neurons Play Opposite Roles in Pathogenesis of Alzheimer's Disease[PubMed: PMC2677455]
Biochemical and Biophysical Research CommunicationsAntagonist of peroxisome proliferator-activated receptor γ induces cerebellar amyloid-β levels and motor dysfunction in APP/PS1 transgenic mice[ScienceDirect: S0006291X09008468]
Electromagnetic Biology and MedicineEffect of 900 MHz Radio Frequency Radiation on Beta Amyloid Protein, Protein Carbonyl, and Malondialdehyde in the Brain[Informa: 15368378.2011.624654]
Electromagnetic Biology and MedicineDo 100- and 500-μT ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains?[PubMed: 23324065]
Indian Journal of Experimental BiologyIntranasal administration of insulin lowers amyloid-levels in rat model of diabetes.[PubMed: 22279939]
European Journal of PharmacologyThe γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1-42 seeding and short-term memory deficits in the Aβ25-35 mouse model of Alzheimer's disease.[ScienceDirect: S0014299912009028]
NeuropsychopharmacologyBlockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease[Pubmed: 23493042]
世界科技研究與發(fā)展(World Sci-tech R & D)慢病毒介導(dǎo)的CBS基因過表達(dá)對(duì)原代皮質(zhì)神經(jīng)元[Globesci:Source]
Neurochemical researchEffects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain[Pubmed:24792732]
Neurochem IntGeniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS1 transgenic model of Alzheimer&#039;s disease[PubMed: 25882165]
Journal of Alzheimer’s Parkinsonism & DementiaThe Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats[open-access:]
Am J Alzheimers Dis Other Demen.Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease[Pubmed:26993489]
Scientific ReportsNorovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s disease[articles:srep41041.ris]
Journal?of?Nutritional?BiochemistryMechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet[Pubmed:29986309]
Journal of Agricultural and?Food?ChemistryEffects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice[Pubmed:29695154]
NeurotoxicologyAmelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats[Pubmed:29522778]
OncotargetDeletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?…[Pubmed:29731975]
Experimental?and?Therapeutic?MedicineEllagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β?amyloid production and tau hyperphosphorylation[Doi: 10.3892/etm.2018.6860]
非編碼RNA miR-137 調(diào)控tau 蛋白磷酸化和L 型鈣離子通道CACNA1C 參與阿爾茨海默病的發(fā)生[]
Neurotoxicity ResearchEffect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 Cells[Pubmed: 30649678]
PLoS OneObstructive sleep apnea may increase the risk of Alzheimer's disease[Pubmed: 31487291]
Frontiers in Aging NeurosciencePrevention of Early Alzheimer's Disease by Erinacine A-Enriched Hericium erinaceus Mycelia Pilot Double-Blind Placebo-Controlled Study[Pubmed: 32581767]
PYRIMIDINE-4H-1OH DERIVATIVES RESTORE MITOCHONDRIAL FUNCTION IN EXPERIMENTAL CHRONIC TRAUMATIC ENCEPHALOPATHY[]
Pharmacognosy MagazineMahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced?…[]
Neurochem IntLovastatin attenuates sevoflurane-induced cognitive disorder in aged rats via reducing A|? accumulation[34048842]
Brain Res BulImmunization with A|?3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ?-[34044034]