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胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

  • 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產品包裝(模擬)
  • 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產品包裝(模擬)
  • 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)實驗結果圖
  • SEA853Hu.jpg標準曲線圖
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測胱天蛋白酶8(CASP8)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

將胱天蛋白酶8(CASP8)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的胱天蛋白酶8(CASP8)與連接于固相載體上的抗體結合，然后加入生物素化的胱天蛋白酶8(CASP8)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的胱天蛋白酶8(CASP8)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

相關產品

編號適用物種：Homo sapiens (Human，人)應用(僅供研究使用，不用于臨床診斷！)
RPA853Hu02胱天蛋白酶8(CASP8)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA853Hu03胱天蛋白酶8(CASP8)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA853Hu01胱天蛋白酶8(CASP8)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA853Hu02胱天蛋白酶8(CASP8)多克隆抗體WB; ICC/IF
PAA853Hu04胱天蛋白酶8(CASP8)多克隆抗體WB; IHC; ICC; IP.
PAA853Hu01胱天蛋白酶8(CASP8)多克隆抗體WB,IHC,ICC/IF
PAA853Hu03胱天蛋白酶8(CASP8)多克隆抗體WB; IHC; ICC/IF
FAA853Hu01抗胱天蛋白酶8(CASP8)多克隆抗體Flow cytometry.
LAA853Hu81胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
LAA853Hu71胱天蛋白酶8(CASP8)多克隆抗體(生物素標記)WB; IHC; ICC.
MAA853Hu21胱天蛋白酶8(CASP8)單克隆抗體WB; IHC
MAA853Hu23胱天蛋白酶8(CASP8)單克隆抗體WB; IHC
MAA853Hu25胱天蛋白酶8(CASP8)單克隆抗體IHC
MAA853Hu24胱天蛋白酶8(CASP8)單克隆抗體WB
MAA853Hu29胱天蛋白酶8(CASP8)單克隆抗體WB
MAA853Hu27胱天蛋白酶8(CASP8)單克隆抗體WB; IHC; ICC; IP.
MAA853Hu22胱天蛋白酶8(CASP8)單克隆抗體WB
SEA853Hu胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA853Hu胱天蛋白酶8(CASP8)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
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參考文獻

雜志參考文獻
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Journal of EthnopharmacologyViscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells[Pubmed:29555410]
Clinical?And Experimental Pharmacology And Physiology?Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway[Pubmed:29266336]
Environmental ToxicologyAntihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice[Pubmed: 31087429]
Environ Toxicol Pharmacol.Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats[Pubmed: 31421311]
Journal of Biochemical and Molecular ToxicologyTranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation[Pubmed: 32045101]
life sciencesNifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats[Pubmed: 32259601]
OdontologyEffects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells[Pubmed: 33206337]
Int ImmunopharmacolCardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling[Pubmed: 33199237]
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGYChemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways[Pubmed: 32942000]
Environ Sci Pollut Res IntPotentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?…[Pubmed: 32915453]
J Biochem Mol ToxicolTranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation[34047436]