細(xì)胞程序性死亡蛋白1配體1(PDL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1)
CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1
- 編號(hào)SEA788Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍0.156-10ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.056ng/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)細(xì)胞程序性死亡蛋白1配體1(PDL1)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的細(xì)胞程序性死亡蛋白1配體1(PDL1)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-101 | 98 |
| EDTA plasma(n=5) | 82-98 | 86 |
| heparin plasma(n=5) | 80-104 | 89 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的細(xì)胞程序性死亡蛋白1配體1(PDL1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中細(xì)胞程序性死亡蛋白1配體1(PDL1)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 84-103% | 99-105% | 79-92% |
| EDTA plasma(n=5) | 94-103% | 97-105% | 99-105% | 99-105% |
| heparin plasma(n=5) | 83-98% | 85-94% | 80-92% | 80-94% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將細(xì)胞程序性死亡蛋白1配體1(PDL1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的細(xì)胞程序性死亡蛋白1配體1(PDL1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的細(xì)胞程序性死亡蛋白1配體1(PDL1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的細(xì)胞程序性死亡蛋白1配體1(PDL1)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA788Hu02 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA788Hu01 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA788Hu61 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA788Hu61 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA788Hu01 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)多克隆抗體 | WB; ICC/IF |
| FAA788Hu01 | 抗細(xì)胞程序性死亡蛋白1配體1(PDL1)多克隆抗體 | Flow cytometry. |
| FAA788Hu41 | 抗細(xì)胞程序性死亡蛋白1配體1(PDL1)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
| FAA788Hu51 | 抗細(xì)胞程序性死亡蛋白1配體1(PDL1)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
| FAA788Hu81 | 抗細(xì)胞程序性死亡蛋白1配體1(PDL1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| MAA788Hu28 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu22 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA788Hu22 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA788Hu21 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu23 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu24 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu25 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu27 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu29 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA788Hu26 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體 | WB; IHC; ICC; IP. |
| RAA788Hu21 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| LAA788Hu72 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| FAA788Hu82 | 抗細(xì)胞程序性死亡蛋白1配體1(PDL1)單克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
| WEA788Hu | 細(xì)胞程序性死亡蛋白1配體1(PDL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA788Hu | 細(xì)胞程序性死亡蛋白1配體1(PDL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA788Hu | 細(xì)胞程序性死亡蛋白1配體1(PDL1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA788Hu | 細(xì)胞程序性死亡蛋白1配體1(PDL1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA788Hu01 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA788Hu01 | 細(xì)胞程序性死亡蛋白1配體1(PDL1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Lancet Haematol | Ratios of T-cell immune effectors and checkpoint molecules as prognostic biomarkers in diffuse large B-cell lymphoma: a population-based study[PubMed: 26686046] |
| Eur J Cancer.? | High levels of the soluble programmed death-ligand (sPD-L1) identify hepatocellular carcinoma patients with a poor prognosis.[Pubmed:27039170] |
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| Allergology International? | Inverse correlation of soluble programmed cell death-1 ligand-1 (sPD-L1) with eosinophil count and clinical severity in allergic rhinitis patients[pubmed:27617656] |
| Medicine | Plasma levels of soluble programmed death ligand-1 may be associated with overall survival in nonsmall cell lung cancer patients receiving thoracic radiotherapy[PMC5319514] |
| American Journal of Respiratory and Critical Care Medicine | Soluble Programed Death Receptor 1 Ligand (sPD-L1) in Pleural Fluid of Patients with Malignant Pleural Mesothelioma (MPM)[Pdf:10.1164] |
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| Immunology?Letters | Increased levels of soluble co-stimulatory molecule PD-L1 (B7-H1) in the plasma of viraemic HIV-1+ individuals[Pubmed: 30236481] |
| Journal of?Cancer?Research?and?Clinical?Oncology | Pre-treatment serum levels of soluble programmed cell death-ligand 1 predict prognosis in patients with hepatitis B-related hepatocellular carcinoma[Pubmed: 30267213] |
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| Scientific Reports | Prognostic implications of soluble programmed death-ligand 1 and its dynamics during chemotherapy in unresectable pancreatic cancer[Pubmed: 31366979] |
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| PLoS One | Clinical implications of APOBEC3A and 3B expression in patients with breast cancer[Pubmed: 32176735] |
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| LIVER INTERNATIONAL | The prognostic role of soluble transforming growth factor‐β and its correlation with soluble programmed death‐ligand 1 in biliary tract cancer[Pubmed: 32780918] |
| CANCER IMMUNOLOGY IMMUNOTHERAPY | Prognostic impacts of tumoral expression and serum levels of PD-L1 and CTLA-4 in colorectal cancer patients[Pubmed: 32577816] |
| Soluble PD-L1: a potential immune marker for HIV-1 infection and virological failure[Pubmed: 32443313] | |
| Cancer Immunol Immunother | The clinical implication of soluble PD-L1 (sPD-L1) in patients with breast cancer and its biological function in regulating the function of T lymphocyte[33688997] |
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