血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Vanin 1 (VNN1)
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
- 編號SEC598Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.059ng/mL.
- 樣本類型serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2940¥ 4200¥ 18900¥ 35700¥ 294000
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測血管非炎性蛋白1(VNN1)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血管非炎性蛋白1(VNN1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-99 | 83 |
| EDTA plasma(n=5) | 80-94 | 89 |
| heparin plasma(n=5) | 82-94 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的血管非炎性蛋白1(VNN1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管非炎性蛋白1(VNN1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 96-105% | 84-94% | 95-103% |
| EDTA plasma(n=5) | 80-101% | 97-105% | 86-94% | 85-95% |
| heparin plasma(n=5) | 78-98% | 88-96% | 91-105% | 83-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將血管非炎性蛋白1(VNN1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的血管非炎性蛋白1(VNN1)與連接于固相載體上的抗體結合，然后加入生物素化的血管非炎性蛋白1(VNN1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血管非炎性蛋白1(VNN1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC598Hu02 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu03 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu01 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu05 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu04 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC598Hu02 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC598Hu04 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC598Hu03 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB |
| PAC598Hu01 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB |
| LAC598Hu71 | 血管非炎性蛋白1(VNN1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAC598Hu24 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB |
| MAC598Hu21 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB |
| MAC598Hu22 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC598Hu | 血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC598Hu | 血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC598Hu01 | 血管非炎性蛋白1(VNN1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology | Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury[ScienceDirect: S0300483X11003234] |
| Journal of Pharmacology and Experimental Therapeutics | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury[Aspet: 192807] |
| J Pharmacol Exp Ther | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury.[PubMed: 22399813] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis[PubMed: 23307618] |
| Iranian Journal of Health and Physical Activity | Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats[Jm.um: Source] |
| Platelets | Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia[Pubmed: 23534352] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis.[Pubmed: 23307618] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats[Sagepub:Source] |
| Food Chem Toxicol. | The effect of activated charcoal on adenine-induced chronic renal failure in rats[Pubmed:24412558] |
| Hypertension Research | Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake[PubMed: 26376947] |
| Inflammation | Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance[PubMed: 26253296] |
| Toxicology | Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma[Pubmed:27317936] |
| J Biomed Mater Res A | Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice[Pubmed:26850371] |
| APMIS | Citrate attenuates vascular calcification in chronic renal failure rats.[pubmed:28332248] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.[pubmed:29027259] |
| Cellular?Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats?…[Pubmed:29550811] |
| International Journal of Molecular?Sciences | Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis[Pubmed: 30332759] |
| International Journal of Molecular Sciences | Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats[Pubmed: 31514290] |
| The Journal of Clinical Hypertension homepage | Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study[Pubmed: 32869948] |
| J Clin Hypertens | Association of urinary vanin?\1 with kidney function decline in hypertensive patients[34029440] |
| Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study[33624223] |
