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Ⅰ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)

NTX-I; NTX1

  • Ⅰ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Ⅰ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Ⅰ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)實驗結(jié)果圖
  • CEA639Mu.jpg標準曲線圖
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測Ⅰ型膠原交聯(lián)氨基端肽(NTXI),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的Ⅰ型膠原交聯(lián)氨基端肽(NTXI)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)80-10182
EDTA plasma(n=5)99-105102
heparin plasma(n=5)82-10290

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型膠原交聯(lián)氨基端肽(NTXI),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中Ⅰ型膠原交聯(lián)氨基端肽(NTXI)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)98-105%88-96%89-96%92-105%
EDTA plasma(n=5)78-98%80-104%95-103%78-105%
heparin plasma(n=5)78-99%78-88%92-101%97-104%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)50µL,
    加入50µL檢測液A(臨用前配制);
    37°C溫育1小時。
3. 洗板3次;
4. 加檢測溶液B100µL,37°C孵育30分鐘;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分鐘;
7. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將Ⅰ型膠原交聯(lián)氨基端肽(NTXI)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本),待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物,然后加入HRP標記的親和素,經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高,標記抗原和抗體的結(jié)合就越受到抑制,顯色愈淺。顯色的深淺與酶量呈正相關(guān),而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關(guān)產(chǎn)品

編號適用物種:Mus musculus (Mouse,小鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
CEA639MuⅠ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
MEA639MuⅠ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA639MuⅠ型膠原交聯(lián)氨基端肽(NTXI)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA639Mu11Ⅰ型膠原交聯(lián)氨基端肽(NTXI)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
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