脂質(zhì)運載蛋白2(NGAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Neutrophil gelatinase-associated lipocalin (NGAL)
Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
- 編號SEB388Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.057ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, urine, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 1966¥ 2808¥ 12636¥ 23868¥ 196560
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測脂質(zhì)運載蛋白2(NGAL)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的脂質(zhì)運載蛋白2(NGAL)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 86-103 | 98 |
| heparin plasma(n=5) | 96-103 | 99 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的脂質(zhì)運載蛋白2(NGAL)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中脂質(zhì)運載蛋白2(NGAL)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-101% | 80-101% | 78-93% | 80-101% |
| EDTA plasma(n=5) | 84-97% | 79-94% | 84-99% | 98-105% |
| heparin plasma(n=5) | 83-98% | 79-101% | 93-101% | 95-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將脂質(zhì)運載蛋白2(NGAL)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的脂質(zhì)運載蛋白2(NGAL)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的脂質(zhì)運載蛋白2(NGAL)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的脂質(zhì)運載蛋白2(NGAL)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB388Mu01 | 脂質(zhì)運載蛋白2(NGAL)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB388Mu61 | 脂質(zhì)運載蛋白2(NGAL)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB388Mu02 | 脂質(zhì)運載蛋白2(NGAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB388Mu61 | 脂質(zhì)運載蛋白2(NGAL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB388Mu01 | 脂質(zhì)運載蛋白2(NGAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB388Mu01 | 脂質(zhì)運載蛋白2(NGAL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB388Mu02 | 脂質(zhì)運載蛋白2(NGAL)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB388Mu81 | 脂質(zhì)運載蛋白2(NGAL)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB388Mu71 | 脂質(zhì)運載蛋白2(NGAL)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEB388Mu | 脂質(zhì)運載蛋白2(NGAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB388Mu | 脂質(zhì)運載蛋白2(NGAL)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB388Mu | 脂質(zhì)運載蛋白2(NGAL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB388Mu01 | 脂質(zhì)運載蛋白2(NGAL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| International Journal of Nephrology and Renovascular Disease | Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy[PubMed: PMC3794962] |
| International Journal of Endocrinology | Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway[Pubmed: 24369464] |
| International Journal of Sport Studies | Lipocalin-2: Response To Resistance Training in Obese Young Men[Ijssjournal: Source] |
| Asian Journal of Sports Medicine | Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men[Tums: Source] |
| European Respiratory Journal | Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap[Pubmed: 23794464] |
| Acta Histochem.? | Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy[Pubmed:24802166] |
| Int Urol Nephrol. | Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental study[Pubmed:25812822] |
| 世界華人消化雜志 | 血清及膽汁中NGAL 對于區(qū)分良惡性膽道狹窄的臨床意義[Wjgnet:Source] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats[Sagepub:Source] |
| Biomedical & Pharmacology Journal | Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men[Biomedpharmajournal:Source] |
| Asian Journal of Physical Education and Computer Science in Sports | A Study On Thyroid Hormone And Its Effects On Exercise [Ifcss:Source] |
| 臨床兒科雜志 | 中性粒細胞明膠酶相關(guān)脂質(zhì)運載蛋白、腎損傷分子1和白介素18 在診斷心肺分流術(shù)后急性腎損傷中的意義[Jcp-Sh:Source] |
| Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy[Pubmed:Pmc4260273] | |
| Int J Chron Obstruct Pulmon Dis. | Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease[Pubmed:Pmc4043430] |
| Pediatric Anesthesia? | Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model[Pubmed:24916275] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis[Pubmed:25448261] |
| Int J Clin Exp Pathol | Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis[PubMed: 26191125] |
| BMC Anesthesiol | Increased cardiac index attenuates septic acute kidney injury: a prospective observational study[PubMed: 25745357] |
| 中華臨床醫(yī)生雜志 | 中性粒細胞明膠酶相關(guān)脂質(zhì)運載蛋白對腹膜透析相關(guān)性腹膜炎療效監(jiān)測的意義[Journal: Fulltext] |
| PLoS One | Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats[PubMed: 25909514] |
| Pediatric Anesthesia | Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model[Pubmed:26714833] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol[publication:299492265_Diabetes-induced_renal_failu] |
| International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats[pubmed:27430552] |
| Hepatology. | Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.[pubmed:27775820] |
| Lipids in Health and Disease | Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN)[pubmed:28659153] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.[pubmed:29027259] |
| Scientific Reports | Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery[Pubmed:29386545] |
| Life?Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity[Pubmed:30036488] |
| Molecules | Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy[Pubmed:29642511] |
| American Journal of Translational?Research | Improved approach for normothermic machine perfusion of cold stored kidney grafts[Pubmed:30018731] |
| Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes[ISBN:978-951-51-4039-5] | |
| Disease Markers | Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis[Doi: 10.1155/2018/1015726] |
| Kidney?and?Blood?Pressure?Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy[Pubmed: 30504713] |
| BMC?Complementary and Alternative Medicine | Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine[Pubmed: 30871515] |
| Naunyn-Schmiedeberg's archives of pharmacology | Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade[Pubmed: 31197431] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation[Pubmed: 31447572] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605] |
| Scientific Reports | Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory Arrest[Pubmed: 31578351] |
| Int Urol Nephrol | The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial?…[Pubmed: 32980930] |
| human & experimental toxicology | Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transition[Pubmed: 32633566] |
| INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК?…[] |
| INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy[] |
| Pediatr Int | Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers[33423326] |
| ARCHIVES OF TOXICOLOGY | Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice[33398418] |
| Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury[33604384] |
| International Journal of Medical Sciences | Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer[34104101] |
| Intensive Care Medicine Experimental | Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental?…[34169407] |
| Biomolecules | Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts[34944524] |
| RSC Adv | SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury[Pubmed:35733690] |
