去甲腎上腺素(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Noradrenaline (NE)
NA; NAd; Norepinephrine; Norepi
- 編號CEA907Ge
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍61.7-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于23.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3342¥ 4774¥ 21481¥ 40576¥ 334152
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測去甲腎上腺素(NE)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的去甲腎上腺素(NE)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-102 | 95 |
| EDTA plasma(n=5) | 97-105 | 101 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的去甲腎上腺素(NE)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中去甲腎上腺素(NE)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-105% | 88-96% | 95-104% | 78-89% |
| EDTA plasma(n=5) | 87-101% | 89-104% | 81-102% | 96-103% |
| heparin plasma(n=5) | 96-104% | 88-96% | 80-98% | 99-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將去甲腎上腺素(NE)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA907Ge11 | 去甲腎上腺素(NE)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA907Ge21 | 去甲腎上腺素(NE)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA907Ge01 | 去甲腎上腺素(NE)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA907Ge81 | 去甲腎上腺素(NE)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA907Ge25 | 去甲腎上腺素(NE)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA907Ge24 | 去甲腎上腺素(NE)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA907Ge23 | 去甲腎上腺素(NE)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA907Ge21 | 去甲腎上腺素(NE)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA907Ge | 去甲腎上腺素(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA907Ge | 去甲腎上腺素(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA907Ge | 去甲腎上腺素(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA907Ge | 去甲腎上腺素(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA907Ge | 去甲腎上腺素(NE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA907Ge11 | 去甲腎上腺素(NE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Renal Failure | Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients[Pubmed: 23530612] |
| Acta Physiologica | Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats[Pubmed:24995933] |
| Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction[PubMed: 25804096] |
| J Cardiovasc Pharmacol Ther | Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 偽1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta.[PubMed: 26025460] |
| Neuropsychiatr Dis Treat | Catha edulis chewing effects on treatment of paranoid schizophrenic patients[PubMed: 25926735] |
| Journal of Caediovascular Pharmacology and Therapeutics | Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta[content:21] |
| Neuropharmacology | Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway[Pubmed:26746861] |
| Microchemical Journal | Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forest[science:S0026265X16300388] |
| Scientific Reports | Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity[pubmed:27694818] |
| PLoS One. | Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice.[pubmed:27598888] |
| Cellular?Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy[pubmed:27889753] |
| European?Journal?of?Trauma?and?Emergency | Xuebijing?injection?treatment?inhibits?vasopermeability?and?reduces?fluid?requirements?in acanine?burn?model.[pubmed:28070608] |
| Acta Physiologica | c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression[pubmed:27439062] |
| Nutrition | Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats[S0899900716302350] |
| Auris Nasus Larynx. | Stress hormonal changes in the brain and plasma after acute noise exposure in mice.[pubmed:27496010] |
| Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling[pubmed:28187460] |
| JOURNAL OF?APPLIED?PHYSIOLOGY? | Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive rats[pubmed:27742806] |
| Nutrition | Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats [pubmed:28241996] |
| Acta Physiologica | Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955] |
| Proceedings of the National Academy of?Sciences?of the United States of America (PNAS) | cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis[Pubmed:29784793] |
| Toxicology?Reports | Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor?…[Pubmed:29854627] |
| International Journal of Nursing?Practice | Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial[Pubmed:29512230] |
| Acta Astronautica | A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction[] |
| Journal of Taibah University for Science | Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles[] |
| Journal of the American College of Cardiology | Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine Model[Pubmed: 31558254] |
| American Journal of Physiology-Heart and Circulatory Physiology | Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive rats[Pubmed: 31834836] |
| European Journal of Pharmacology | Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats[Pubmed: 31811858] |
| A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injury[Pubmed: 31876952] | |
| The American Journal of Pathology | Sympathetic Nerves Positively Regulate Eosinophil-Driven Allergic Conjunctivitis via α1-Adrenergic Receptor Signaling[Pubmed: 32194050] |
| pharmaceutical biology | Role of melatonin in intestinal mucosal injury induced by restraint stress in mice[Pubmed: 32298156] |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis[Pubmed: 33177714] |
| European Journal of Medical Research | Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine model[Pubmed: 32552871] |
| Scientific Reports | Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation[Pubmed: 32332859] |
| bioRxiv | Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation[] |
| Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair?…[] | |
| Cogent Medicine | Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides[] |
| PHARMACOLOGICAL RESEARCH | Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2[33352230] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice[33303990] |
| Mol Metab | Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D[33549845] |
| International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ?-[33762821] |
| Int J Hypertens | The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models[33884205] |
| Kidney Blood Press Res | Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery[34852339] |
| Hypertension | Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy?…[Pubmed:35189708] |
| Exp Eye Res | Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion[Pubmed:35421396] |
