骨膜蛋白(POSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Periostin (POSTN)
OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2
- 編號SEH339Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.055ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測骨膜蛋白(POSTN)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的骨膜蛋白(POSTN)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 95 |
| EDTA plasma(n=5) | 91-101 | 96 |
| heparin plasma(n=5) | 84-99 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的骨膜蛋白(POSTN)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中骨膜蛋白(POSTN)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-102% | 88-96% | 80-105% | 83-101% |
| EDTA plasma(n=5) | 87-97% | 78-103% | 89-97% | 91-98% |
| heparin plasma(n=5) | 94-102% | 79-101% | 86-94% | 88-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將骨膜蛋白(POSTN)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的骨膜蛋白(POSTN)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的骨膜蛋白(POSTN)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的骨膜蛋白(POSTN)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPH339Mu01 | 骨膜蛋白(POSTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH339Mu02 | 骨膜蛋白(POSTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH339Mu02 | 骨膜蛋白(POSTN)多克隆抗體 | WB |
| PAH339Mu03 | 骨膜蛋白(POSTN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAH339Mu01 | 骨膜蛋白(POSTN)多克隆抗體 | WB |
| FAH339Mu01 | 抗骨膜蛋白(POSTN)多克隆抗體 | FCM |
| LAH339Mu71 | 骨膜蛋白(POSTN)多克隆抗體(生物素標(biāo)記) | WB |
| LAH339Mu81 | 骨膜蛋白(POSTN)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| SEH339Mu | 骨膜蛋白(POSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH339Mu | 骨膜蛋白(POSTN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Blood | Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients; A Novel Marker Of Bone Strength?[Bloodjournal: Source] |
| The Journal of Clinical Endocrinology & Metabolism | Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY study[Pubmed: 24628551] |
| J Clin Endocrinol Metab. | Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY Study[Pubmed:24628551] |
| Rheumatology (Oxford). | Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation[Pubmed:25349442] |
| Journal of Periodontal Research | Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease[Pubmed:25529858] |
| 新醫(yī)學(xué) | 血清內(nèi)脂素水平與多囊卵巢綜合征的相關(guān)性研究[Xinyixue:Source] |
| Osteoarthritis | Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study[PubMed: 26072384] |
| Endocrine | Serum periostin is a potential biomarker for non-alcoholic fatty liver disease: a case– control study[PubMed: 26362060] |
| Blood?Cancer?Journal | High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly …[pubmed:27716740] |
| Metabolism.? | Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity.[pubmed:28521873] |
| Osteoporosis International | Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older women[pubmed:28382553] |
| Molecular Diagnosis & Therapy | The Utility of Biomarkers in Osteoporosis Management[pubmed:28271451] |
| Journal of Bone and Mineral Research | Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missions[doi:10.1002] |
| Clinical Cases in Mineral and Bone Metabolism | Periostin and sclerostin levels in juvenile Paget's disease[pubmed:29263750] |
| Calcified?Tissue?International | Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bone[pubmed:28725907] |
| Wiley | Characterization of a sandwich ELISA for the quantification of all human periostin isoforms[pubmed:28493527] |
| International Osteoporosis Foundation and National Osteoporosis Foundation | Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructure[pubmed:29038835] |
| Bone | Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormones[pubmed:28323143] |
| Clinica Chimica Acta | Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhage[pubmed:28882488] |
| Clinica Chimica Acta | Serum periostin concentrations and outcomes after severe traumatic brain injury[pubmed:28668564] |
| Tumor Biology | Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapy[pubmed:28459197] |
| Journal of Bone and Mineral Research | Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAX[pubmed:28766739] |
| ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ[2017; 62:(4)] | |
| Endocrine | Circulating periostin in patients with nonalcoholic fatty liver disease.[pubmed:27738886] |
| Molecular Medicine Reports | Periostin cross?reacts with the renin?angiotensin system during liver fibrosis development[pubmed:28849131] |
| The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice[Pubmed:29929978] |
| Calcified?Tissue?International | The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone Metastasis[Pubmed:29916127] |
| Journal of Endocrinological Investigation | Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus[Pubmed:29349642] |
| Wiener klinische Wochenschrift | Rheumatoid arthritis in remission[] |
| Clinical?and Experimental Medicine | Myostatin and other musculoskeletal markers in lung transplant recipients[Pubmed: 30317402] |
| Experimental?and?Clinical?Endocrinology?&?Diabetes | Polycystic Ovary Syndrome is Associated with Elevated Periostin Levels[Pubmed: 30372763] |
| ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ[] | |
| Bone | Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bone[Pubmed: 31051316] |
| Journal of Clinical Laboratory Analysis | Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancer[Pubmed: 31268191] |
| Bone | Serum periostin is associated with cancer mortality but not cancer risk in older home-dwelling men: A 8-year prospective analysis of the STRAMBO study[Pubmed: 31812700] |
| FASEB J | Fibronectin rescues aberrant phenotype of endothelial cells lacking either CCM1, CCM2 or CCM3[Pubmed: 32515053] |
| Journal of Reproductive Immunology | Serum and placental periostin levels in women with early pregnancy loss[Pubmed: 32460058] |
| Annals of Medical Research | Evaluation of periostin levels in gingival crevicular fluid and peri-implant sulcus fluid in patients with periodontal and peri-implanter disease: A cross-sectional?…[] |
| Myostatin and Markers of Bone Metabolism in Dermatomyositis[] | |
| Am J Physiol Heart Circ Physiol | Periostin promotes arterial calcification through PPAR|?-related glucose metabolism reprogramming[33834866] |
| International Journal of Chronic Obstructive Pulmonary Disease | High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease[33814903] |
| J Bone Oncol | Serum total periostin is an independent marker of overall survival in bone metastases of lung adenocarcinoma[34150488] |
| Rambam Maimonides Med J | The Relationship between Serum Periostin Levels and Neutrophil¨CLymphocyte Ratio with Lesion Localization and Acute Prognosis in Acute Ischemic Stroke[34137680] |
| Rambam Maimonides Medical Journal | First Admission Neutrophil–Lymphocyte Ratio May Indicate Acute Prognosis of Ischemic Stroke[34137680] |
