免疫球蛋白G(IgG)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Immunoglobulin G (IgG)
- 編號CEA544Rb
- 物種Oryctolagus cuniculus (Rabbit，兔)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍12.35-1,000ug/mL
- 靈敏度最小可檢測劑量小于等于4.61ug/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 1756¥ 2508¥ 11286¥ 21318¥ 175560
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測免疫球蛋白G(IgG)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白G(IgG)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-101 | 79 |
| EDTA plasma(n=5) | 96-104 | 99 |
| heparin plasma(n=5) | 89-99 | 94 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的免疫球蛋白G(IgG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白G(IgG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-94% | 96-104% | 87-105% | 87-101% |
| EDTA plasma(n=5) | 89-102% | 97-105% | 95-105% | 96-105% |
| heparin plasma(n=5) | 92-99% | 88-96% | 91-102% | 96-104% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板5次；
4. 加TMB底物90µL，37°C孵育10-20分鐘；
5. 加終止液50µL，立即450nm讀數。
實驗原理
本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將免疫球蛋白G(IgG)抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入過氧化物酶標記的抗原和待測抗原(標準品或樣本)，待測抗原與酶標記抗原對特異性抗體進行競爭結合。經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| NPA544Rb01 | 免疫球蛋白G(IgG)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA544Rb62 | 免疫球蛋白G(IgG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA544Rb61 | 免疫球蛋白G(IgG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SAA544Rb10 | 山羊抗兔IgG多克隆抗體(無標記) | WB |
| PAA544Rb51 | 免疫球蛋白G(IgG)多克隆抗體 | WB; IHC |
| SAA544Rb14 | 山羊抗兔IgG多克隆抗體(藻紅素標記) | WB; IHC; ICC; IF. |
| SAA544Rb50 | 豚鼠抗兔IgG多克隆抗體(無標記) | WB; IP; IHC-P; IHC-F; ELISA. |
| SAA544Rb16 | 山羊抗兔IgG多克隆抗體(吲哚菁綠標記) | WB; IHC; ICC; IF. |
| SAA544Rb56 | 豚鼠抗兔IgG多克隆抗體(吲哚菁綠標記) | WB; IHC; ICC; IF. |
| SAA544Rb58 | 豚鼠抗兔IgG多克隆抗體(異硫氰酸熒光素標記) | ICC/IF |
| SAA544Rb15 | 山羊抗兔IgG多克隆抗體(別藻藍蛋白標記) | WB; IHC; ICC; IF. |
| SAA544Rb17 | 山羊抗兔IgG多克隆抗體(生物素標記) | WB; IHC; ICC; IF. |
| SAA544Rb18 | 山羊抗兔IgG多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| SAA544Rb19 | 山羊抗兔IgG多克隆抗體(辣根過氧化物酶標記) | WB |
| SAA544Rb54 | 豚鼠抗兔IgG多克隆抗體(藻紅素標記) | WB; IHC; ICC; IF. |
| SAA544Rb29 | 小鼠抗兔IgG多克隆抗體(辣根過氧化物酶標記) | WB; IHC; ICC. |
| SAA544Rb20 | 小鼠抗兔IgG多克隆抗體(無標記) | ELISA;WB; IHC; ICC;IF. |
| SAA544Rb57 | 豚鼠抗兔IgG多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SAA544Rb55 | 豚鼠抗兔IgG多克隆抗體(別藻藍蛋白標記) | WB; IHC; ICC; IF. |
| SAA544Rb59 | 豚鼠抗兔IgG多克隆抗體(辣根過氧化物酶標記) | WB; IHC |
| MAA544Rb26 | 免疫球蛋白G(IgG)單克隆抗體 | IHC |
| MAA544Rb27 | 免疫球蛋白G(IgG)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA544Rb29 | 免疫球蛋白G(IgG)單克隆抗體 | IHC |
| MAA544Rb21 | 免疫球蛋白G(IgG)單克隆抗體 | WB; IHC |
| MAA544Rb22 | 免疫球蛋白G(IgG)單克隆抗體 | IHC |
| MAA544Rb23 | 免疫球蛋白G(IgG)單克隆抗體 | IHC |
| MAA544Rb24 | 免疫球蛋白G(IgG)單克隆抗體 | IHC |
| MAA544Rb25 | 免疫球蛋白G(IgG)單克隆抗體 | IHC |
| CEA544Rb | 免疫球蛋白G(IgG)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA544Rb | 免疫球蛋白G(IgG)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA544Rb | 免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA544Rb11 | 免疫球蛋白G(IgG)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine[Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus[Medwell: 2010594600] |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI[Ijfans: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88[Pubmed: 24570422 ] |
| Pharmaceutical Development and Technology | Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics[Pubmed: 23590209] |
| BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling[Hindawi:Source] |
| Journal of Proteomics | Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection[Pubmed:24576640] |
| Int J Occup Environ Health. | Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors[Pubmed:24999850] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice[Pubmed:25308407] |
| Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer[Pubmed:25529861] |
| Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling[PubMed: 26161394] |
| Protein Pept Lett | Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres[PubMed: 26268563] |
| Electrophoresis | Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27[Pubmed:26914286] |
| Neuropeptides | Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro.[Pubmed:26707636] |
| Archives of Biological Sciences | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine[pid:46641600021Z] |
| Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS[journals:3790] |
| journal of biotechnology | Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans.[pubmed:28013072] |
| Sci Rep.? | Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.[pubmed:28220870] |
| Toxicology Research | Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis[C6TX00288A] |
| ntervirology | Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide[pubmed:27694750] |
| Biomedicine?&?Pharmacotherapy | Icariin?promotes?osteogenic?differentiation?of?rat?bone?marrow?stromal?cells?by?activating?the ERα-Wnt/β-catenin?signaling?pathway.[pubmed:27764755] |
| Food and Agricultural Immunology | Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers[10.108:09540105.2017.1311308 ] |
| Biomedicine & Pharmacotherapy | In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats[pubmed:29091887] |
| Journal of Biochemical and Molecular Toxicology | Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein[10.1002/jbt.21912] |
| International Journal of Science and Research | Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria[ISSN: 2319-7064] |
| Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt.[pubmed:28586019] |
| Revista Brasileira de Zootecnia | Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders[10.1590/s1806-92902017000200004?] |
| Water?Research? | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model[Pubmed:29655095] |
| Clinical?Immunology | Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis[Pubmed:30056130] |
| Food and Agricultural Immunology | Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers[10.1080:09540105.2018.1457013] |
| Journal of Molecular Neuroscience | A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related Enzymes[Pubmed:30062438] |
| Biological?Trace?Element?Research | Supplementation of Cr Methionine During Dry Period of Dairy Cows and Its Effect on Some Production and Biochemical Parameters During Early Lactation and on?…[Pubmed:29497999] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a?…[Pubmed:29305686] |
| SAGE?Open?Medicine | Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes[Pubmed:29623201] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids[Pubmed: 30237167] |
| ACS?Applied?Materials?&?Interfaces | Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications[Pubmed: 30433758] |
| Molecular?Human?Reproduction | Phenotypic variations in transferred progeny due to genotype of surrogate mother: Surrogate mother modulates transferred progeny[Pubmed: 30445548] |
| Bioscience, Biotechnology, and Biochemistry | In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6[] |
| Journal?of?proteome?research | Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection[] |
| International Immunopharmacology | Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice[Pubmed: 30974284] |
| Utjecaj dodatka selena u prihrani na hematolo?ku sliku i biokemijske pokazatelje u jelena lopatara (Dama dama L.)[] | |
| Biogerontology | Age-related chemokine alterations affect IgA secretion and gut immunity in female mice[Pubmed: 32277312] |
| International Journal of Biological Macromolecules | Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens[Pubmed: 32224178] |
| Pharmaceutics | Efficacy of Local Minocycline Agents in Treating Peri-Implantitis: An Experimental In Vivo Study in Beagle Dogs[Pubmed: 33114168] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharide[Pubmed: 33010421] |
| Pharmaceutics | Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo Study[Pubmed: 32708741] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle[Pubmed: 32583225] |
| Molecular Medicine Reports | Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3?E1 cells via an autocrine mechanism[Pubmed: 33000198] |
| South African Journal of Botany | Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo[] |
| Journal of Functional Foods | Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts[] |
| Italian Journal of Animal Science | Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the?…[] |
| Regenerative Biomaterials | A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model[] |
| ACS APPLIED BIO MATERIALS | Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy[] |
| 博士論文 | Women Scientist Scientist Mentor[] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate[] |
| THERANOSTICS | Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without??-[33456566] |
| Life Sci | Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats[33577848] |
| Animals (Basel) | Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 404[33562568] |
| Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure[34090073] |
| Veterinary Microbiology | Optimized GAPDH-truncated immunogen of Streptococcus equi elicits an enhanced immune response and provides effective protection in a mouse model[33647714] |
| Experimental and Therapeutic Medicine | Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats[33574912] |
| British Journal of Pharmacology | The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus[] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle[] |
| Neonatal Buza??larda ?ki Farkl? ?mmunomod¨1lat?r¨1n Buza?? ?shalleri ¨1zerine Etkileri[] | |
| Microbiology Spectrum | Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows[34494854] |
| Pediatrics International | Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus[34559910] |
| Toxicology | Protection from Benzene-induced Immune Dysfunction in Mice[Pubmed:35090963] |
| Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components[Pubmed:35438093] |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death[Pubmed:35732625] |
| Nature Communications | Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction[Pubmed:35668082] |
| Food and Agricultural Immunology | The effect of the multi-strain probiotic preparation EM Bokashi? on selected parameters of the cellular immune response in pigs[] |
| 陸軍軍醫(yī)大學學報 | 信筒子醌對系統性紅斑狼瘡小鼠免疫調節(jié)和治療作用[] |
