視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- 編號SEA929Ga
- 物種Chicken (Gallus，雞)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.071ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測視黃醇結合蛋白4(RBP4)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的視黃醇結合蛋白4(RBP4)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-102 | 95 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 87-96 | 92 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的視黃醇結合蛋白4(RBP4)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中視黃醇結合蛋白4(RBP4)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-103% | 82-102% | 81-95% | 98-105% |
| EDTA plasma(n=5) | 98-105% | 82-102% | 96-103% | 93-101% |
| heparin plasma(n=5) | 86-102% | 84-95% | 84-93% | 90-105% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將視黃醇結合蛋白4(RBP4)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的視黃醇結合蛋白4(RBP4)與連接于固相載體上的抗體結合，然后加入生物素化的視黃醇結合蛋白4(RBP4)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的視黃醇結合蛋白4(RBP4)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA929Ga01 | 視黃醇結合蛋白4(RBP4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA929Ga01 | 視黃醇結合蛋白4(RBP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA929Ga01 | 視黃醇結合蛋白4(RBP4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA929Ga71 | 視黃醇結合蛋白4(RBP4)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA929Ga81 | 視黃醇結合蛋白4(RBP4)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| SEA929Ga | 視黃醇結合蛋白4(RBP4)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA929Ga | 視黃醇結合蛋白4(RBP4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA929Ga01 | 視黃醇結合蛋白4(RBP4)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078] |
| Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2[PubMed: 21130593] |
| Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats[PubMed: PMC3368504] |
| Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles[Pubmed:24501381] |
| J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats[Pubmed:25240061] |
| Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats[Pubmed:25445608] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats[Pubmed:25697126] |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome[PubMed: 26351173] |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-??B in diabetic rats with atherosclerosis and the intervention effect of pioglitazone[Pubmed:27446311] |
| Experimental and Therapeutic Medicine | Expression?of?retinol?binding?protein?4?and?nuclear?factor-κB in?diabetic?rats?with?atherosclerosisand the?intervention?effect?of?pioglitazone.[pubmed:27446311] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis[Pubmed:29386009] |
| Nutrition &?Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans[Pubmed:29335416] |
| Molecular?Medicine?Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway[Pubmed:30015949] |
| Relationship between retinol and risk of diabetic retinopathy: a case-control study[] | |
| Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle[Pubmed: 31147589] |
| Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals[] | |
| Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma[] | |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren[33575861] |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren[33834274] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats[33833309] |
| Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers[34773507] |
