中性粒細(xì)胞彈性蛋白酶(NE)真核蛋白
Eukaryotic Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- 編號(hào)EPA181Mu61
- 物種Mus musculus (Mouse,小鼠)相同的名稱,不同的物種。
- 來源真核表達(dá)
- 宿主293F Cell
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞質(zhì), 細(xì)胞外基質(zhì)
- 預(yù)測(cè)分子量21.7kDa
- 實(shí)際分子量33kDa(差異分析請(qǐng)參閱說明書)
- 片段與標(biāo)簽Ser27~Asn265 with N-terminal His Tag
- 緩沖液成份磷酸鹽緩沖液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)9.6
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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序列

用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APA181Mu61 | 中性粒細(xì)胞彈性蛋白酶(NE)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA181Mu01 | 中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA181Mu02 | 中性粒細(xì)胞彈性蛋白酶(NE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA181Mu61 | 中性粒細(xì)胞彈性蛋白酶(NE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA181Mu01 | 中性粒細(xì)胞彈性蛋白酶(NE)多克隆抗體 | WB; IHC; ICC; IP. |
PAA181Mu02 | 中性粒細(xì)胞彈性蛋白酶(NE)多克隆抗體 | WB; IHC; ICC; IP. |
SEA181Mu | 中性粒細(xì)胞彈性蛋白酶(NE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA181Mu | 中性粒細(xì)胞彈性蛋白酶(NE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
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Bioscience Reports | Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats[Pubmed: 31763669] |
Oxidative Medicine and Cellular Longevity | Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation[Pubmed: 31871555] |
Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis[Pubmed: 31927460] |
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Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload?\Related Liver Ischemia/Reperfusion Injury[33959955] |
Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling??-[33505502] |
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Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome[Pubmed:35088760] |
A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation[] |