可羅索(KL)重組蛋白
Recombinant Klotho (KL)
KLa; Klotho Alpha
- 編號RPH757Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位細(xì)胞膜, 分泌
- 預(yù)測分子量54.8kDa
- 實際分子量55&54kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Pro516~Phe955 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點6.8
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 912¥ 2280¥ 4560¥ 13680¥ 34200
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPH757Mu03 | 可羅索(KL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Mu02 | 可羅索(KL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Mu01 | 可羅索(KL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH757Mu02 | 可羅索(KL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAH757Mu01 | 可羅索(KL)多克隆抗體 | WB; IHC; ICC; IP. |
| LAH757Mu71 | 可羅索(KL)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAH757Mu81 | 可羅索(KL)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| SEH757Mu | 可羅索(KL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEH757Mu | 可羅索(KL)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCH757Mu | 可羅索(KL)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMH757Mu | 可羅索(KL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSH757Mu01 | 可羅索(KL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Life Sciences | High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature[Pubmed: 24012609] |
| EXPERIMENTAL & CLINICAL CARDIOLOGY | Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice[Com:Source] |
| Int J Clin Exp Pathol.? | α-Klotho is an acute phase protein and altered by restraint stress in mice[Pubmed:Pmc4203207] |
| Annales de Biologie Clinique | Les biomarqueurs des calcifications vasculaires[PubMed: 26069069] |
| Mol Med Rep | Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho[PubMed: 25873020] |
| Calcif Tissue Int | Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3[PubMed: 25899975] |
| 中華內(nèi)分泌代謝雜志 | 抗衰老蛋白Klotho 阻斷高糖培養(yǎng)的大鼠腎小球系膜細(xì)胞TLR4/NF-κB p65/NGAL 通路的激活及其作用機(jī)制[Cn: Article] |
| Mol Cell Endocrinol | The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism[PubMed: 26419931] |
| Arch Med Sci | Soluble Klotho protein as a novel serum biomarker in patients with acromegaly[pmc:PMC4754356] |
| Am J Nephrol | Klotho?Deficiency?Aggravates?Tacrolimus-Induced?Renal?Injury?via?the?Phosphatidylinositol?3-Kinase-Akt-Forkhead?Box?Protein?O?Pathway[Pubmed:27174564] |
| Sci Rep. | Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice.[pubmed:28387374] |
| Nephrology | Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?[pubmed:28429560] |
| Journal of Integrative Nephrology & Andrology | Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients[issn:2394-2916] |
| International Journal of Clinical and Experimental Pathology | Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice[ISSN:1936-2625] |
| Journal of veterinary medical science | Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model[pubmed:28943597] |
| Cellular Physiology and Biochemistry | Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury[Pubmed:29550818] |
| American Journal of Translational?Research | Advanced maternal age impairs spatial learning capacity in young adult mouse offspring[Pubmed:29636887] |
| Journal of?Diabetes?and its?Complications | Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications[Pubmed:30042059] |
| Medical?Science?Monitor | Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal?…[Pubmed:30004089] |
| Nephrology Dialysis?Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease[Pubmed: 30189026] |
| BMC?Nephrology | The role of klotho in chronic kidney disease[Pubmed: 30348110] |
| Nature?Communications | Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration[Pubmed: 30451844] |
| Kidney?International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome[Pubmed: 30770217] |
| Expert?Opinion on?Therapeutic?Targets | The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats[Pubmed: 30801205] |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection[Pubmed: 33223223] |
| Journal of Clinical Hypertension | Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity[Pubmed: 33164306] |
| CURRENT EYE RESEARCH | Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats[Pubmed: 32744464] |
| international immunopharmacology | Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats[Pubmed: 32438076] |
| Purinergic Signalling | P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy.[Pubmed: 32377919] |
| Oxidative Medicine and Cellular Longevity | Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury[Pubmed: 33123314] |
| NEUROSCIENCE | Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia[Pubmed: 32612508] |
| Pharmacological Reports | |áKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy[33675964] |
| J Mol Neurosci | Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells[Pubmed:35112226] |
| Reproductive Biology and Endocrinology | Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance[] |
| Journal of Ovarian Research | Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome[] |