降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Procalcitonin (PCT) ,etc. by FLIA (Flow Luminescence Immunoassay)
Pro-Calcitonin
（注：單次混測多因子不超過8個指標 ）
- 編號LMA689Rb
- 物種Oryctolagus cuniculus (Rabbit，兔)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍1.9531-2000pg/mL
- 靈敏度最小可檢測劑量小于等于0.651pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 78-97 | 85 |
| heparin plasma(n=5) | 91-99 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-92% | 78-101% | 83-93% | 82-95% |
| EDTA plasma(n=5) | 81-93% | 95-103% | 96-104% | 87-102% |
| heparin plasma(n=5) | 86-93% | 82-98% | 78-102% | 81-92% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。
贈品
相關產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPA689Rb61 | 降鈣素原(PCT)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CEA689Rb | 降鈣素原(PCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA689Rb | 降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Scandinavian Journal of Infectious Diseases | Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis[PubMed: 20085423] |
| International Journal of Radiation Oncology ? Biology ? Physics | Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice[PubMed: 20637981] |
| Ulus Travma Acil Cerrahi Derg | Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia[PubMed: 21935794] |
| Egyptian Journal of Bronchology | Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children[Essbronchology: Source] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source] |
| Egyptian Journal of Medical Microbiology | Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis[Ejmm:Source] |
| Nigerian Medical Journal | Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis[Pubmed:25657488] |
| Wiley online library | The use and value of procalcitonin in solid organ transplantation[PubMed: 25996831] |
| Balkan Med J | Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia[PubMed: 26185718] |
| The Journal of Clinical Endocrinology & Metabolism | Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay[PubMed: 26647152] |
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| Cellular?Physiology and Biochemistry | Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure[Pubmed:29913443] |
| Archives of Medical Science | Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase[Pubmed:29593801] |
| Journal of Thoracic Disease | Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung[Pubmed:29707313] |
| Asian?Journal of Psychiatry | Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients[Pubmed:29723719] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.[Pubmed: 31298341] |
| Medicina | Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection[Pubmed: 33080909] |
| MEDIATORS OF INFLAMMATION | Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase[Pubmed: 32410851] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes[] | |
| Journal of Immunology Research | Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis[33628853] |
| Journal of Medical Biochemistry | Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer[33776564] |
| Surg Endosc | Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity[Pubmed:35044516] |
| Bone Res | Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment[Pubmed:35087025] |