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內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Endothelial Cell Specific Molecule 1 (ESM1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Endocan

（注：單次混測多因子不超過8個指標 ）

  • 內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)87-10191
EDTA plasma(n=5)96-10399
heparin plasma(n=5)79-10190

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)95-104%85-101%96-103%84-91%
EDTA plasma(n=5)86-96%79-91%79-102%98-105%
heparin plasma(n=5)79-91%89-97%84-95%80-101%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號適用物種：Mus musculus (Mouse，小鼠)應用(僅供研究使用，不用于臨床診斷！)
RPC463Mu01內(nèi)皮細胞特異分子1(ESM1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPC463Mu02內(nèi)皮細胞特異分子1(ESM1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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PAC463Mu02內(nèi)皮細胞特異分子1(ESM1)多克隆抗體WB; IHC; ICC; IP.
SEC463Mu內(nèi)皮細胞特異分子1(ESM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC463Mu內(nèi)皮細胞特異分子1(ESM1)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
UrologyDetection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation[PubMed: 22608803]
Microvasc Res. Endocan is useful biomarker of survival and severity in sepsis.[Pubmed: 24769132]
J Matern Fetal Neonatal Med.Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes[Pubmed:25211383]
Microvasc Res.Endocan is useful biomarker of survival and severity in sepsis[Pubmed:24769132]
AngiologyEndocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus[PubMed: 26614790]
Archives of Medical Science - Atherosclerotic DiseasesAssessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study[Journal:-102]
Int Immunopharmacol.?Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension.[pubmed:27912147]
OncotargetDiagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancer[pubmed:28514746]
Biomedical and Pharmacology Journal Changes in Endocan Levels and Blood Coagulation in HIV Infection[DOI: 10.13005/bpj/1082]
Life SciencesA novel role of endocan in alleviating LPS-induced acute lung injury[Pubmed:29627442]
International Urology and NephrologyIs the plasma endocan level a reliable predictor for the severity of erectile dysfunction?[Pubmed:30054863]
The Open Rheumatology JournalSerum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity[Doi: 10.2174/1874312901812010189]
Molecular?Medicine?ReportsDownregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor?κB activity[Pubmed: 30816462]
Biomarkers in MedicineEndothelial biomarkers in the light of new sepsis definition[Pubmed: 30920847]
Experimental and Therapeutic MedicineRisk stratification and prognostic evaluation of endothelial cell?specific molecule1, von Willebrand factor, and a disintegrin?like and metalloprotease with?…[]
Turkish Journal of Medical SciencesA novel marker relationship between carotid intima–media thickness and disease activity score-28 in patients with rheumatoid arthritis: human endothelial cell?…[Pubmed: 31655533]
BiomarkersRole of Ascitic Endocan Levels in the Diagnosis of Spontaneous Bacterial Peritonitis in Decompensated Cirrhosis[Pubmed: 32364003]
J Obstet Gynaecol ResComparison of the maternal serum endocan levels in preterm premature rupture of membrane and normal pregnancy[34109715]
turkish journal clinics laboratoryThe relationship between serum endocan levels with the presence of contrast-induced nephropathy in patients undergoing coronary angiography[]