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膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay)

ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

（注：單次混測多因子不超過8個(gè)指標(biāo) ）

  • 膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)90-10297
EDTA plasma(n=5)90-9794
heparin plasma(n=5)86-9692
sodium citrate plasma(n=5)79-10487

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)90-103%80-90%97-104%80-105%
EDTA plasma(n=5)82-102%81-104%84-96%93-101%
heparin plasma(n=5)85-99%90-97%93-101%85-101%
sodium citrate plasma(n=5)94-102%81-97%90-98%86-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈(zèng)品

相關(guān)產(chǎn)品

編號(hào)適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
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LMB944Mu膜聯(lián)蛋白A2(ANXA2)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志參考文獻(xiàn)
Journal of Cellular BiochemistrySubcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis[PubMed: 20225235]
World Journal of GastroenterologyExpression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma[PubMed: PMC3491596]
Oncology LettersAnnexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis[PubMed: PMC3742823]
IbrahimAnnexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma[Ghrnet: Source]
European Journal of Clinical Microbiology &amp; Infectious DiseasesSerum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis[Pubmed:24853056]
Biomedicine &amp; PharmacotherapyCirculating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables[Pubmed:25661364]
Hepat MonSerum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B[PubMed: 26587036]
Tumour BiolUp-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma[PubMed: 26109000]
Biomed PharmacotherCirculating[PubMed: 25661364]
Tumour BiolEvaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma[PubMed: 26189841]
Eur J Clin InvestStairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population[PubMed: 26081791]
Journal of Oral and Maxillofacial SurgerySerum?Annexin?A2?Level?Is?Associated?With?Diagnosis?and?Prognosis?in?Patients?With?OralSquamous?Cell?Carcinoma.[pubmed:27889534]
Pregnancy HypertensAssessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study.[pubmed:28501283]
Mol Nutr Food Res.Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells.[pubmed:28181408]
Archives of Gynecology and ObstetricsExpression of annexin A2 in adenomyosis and dysmenorrhea[Pubmed: 31183557]
CancersExtracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer[Pubmed: 31842290]
PLASMA BIOMARKER FOR OVARIAN CANCER[]
DiagnosticsDiagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69[33406648]
ADVANCED RESEARCHESSearch for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[]
Cancer Cell InternationalBufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[]
Cancer Cell IntBufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212]