男人女人午夜视频免费,免费人妻无码不卡中文字幕系,国产A级三级三级三级,成年无码aⅴ片在线观看

心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Atrial Natriuretic Peptide (ANP) ,etc. by FLIA (Flow Luminescence Immunoassay)

ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin

（注：單次混測多因子不超過8個指標 ）

  • 心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)產品包裝(模擬)
  • 心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)產品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)80-10187
EDTA plasma(n=5)83-9487
heparin plasma(n=5)93-10297

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)96-103%92-101%92-101%90-99%
EDTA plasma(n=5)92-103%82-102%79-101%90-101%
heparin plasma(n=5)87-102%93-105%78-93%78-94%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數。

實驗原理

將心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。

贈品

相關產品

編號適用物種：Rattus norvegicus (Rat，大鼠)應用(僅供研究使用，不用于臨床診斷！)
RPA225Ra01心鈉肽(ANP)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA225Ra02心鈉肽(ANP)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA225Ra03心鈉肽(ANP)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA225Ra02心鈉肽(ANP)多克隆抗體WB; IHC; ICC; IP.
PAA225Ra03心鈉肽(ANP)多克隆抗體WB; IHC; ICC; IP.
PAA225Ra01心鈉肽(ANP)多克隆抗體WB; IHC; ICC; IP.
LAA225Ra71心鈉肽(ANP)多克隆抗體(生物素標記)WB; IHC; ICC.
LAA225Ra81心鈉肽(ANP)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
CEA225Ra心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
MEA225Ra心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA225Ra心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA225Ra01心鈉肽(ANP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
8Volume Status and Diuretic Therapy in Systolic Heart Failure and the Detection of Early Abnormalities in Renal and Tubular Function[PubMed: 21616283]
Basic Research in Cardiology Release of atrial natriuretic peptide precedes shedding of the endothelial glycocalyx equally in patients undergoing on- and off-pump coronary artery bypass surgery[PubMed: 21769675]
Neuropsychobiology. Atrial natriuretic Peptide, arginine vasopressin Peptide and cortisol serum levels in opiate-dependent patients[PubMed: 23406607]
European addiction researchAlcohol-induced changes in methylation status of individual CpG sites, and serum levels of vasopressin and atrial natriuretic peptide in alcohol-dependent patients during detoxification treatment.[Pubmed: 24356727]
Crit Care.Hypervolemia increases release of atrial natriuretic peptide and shedding of the endothelial glycocalyx[Pubmed:25497357]
Diabet Med.?Sodium-glucose co-transporter-2 inhibitors suppress atrial natriuretic peptide secretion in patients with newly diagnosed Type 2 diabetes.[Pubmed:26927445]
J EthnopharmacolDanhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway[Pubmed:26970569]
Journal of Babylon University/Pure and Applied SciencesBlood Pressure and Atrial Natriuretic Peptide (ANP) Levels in Patients with Autosomal Dominant Polycystic Kidney Disease.[papers:uobj_paper_2016_62613672.pdf]
PLoS OneEnhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy[pubmed:29240788]
Drug DeliveryGinsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy.[pubmed:29063791]
ElifeSIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation[Pubmed:29504933]
Science?of the?Total?EnvironmentCo-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and[Pubmed:29727991]
The Journal of Biological ChemistryToll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice[Pubmed:29929978]
Pediatric CardiologyDeterminants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease[Pubmed:29392348]
FASEB?JournalSignature-oriented investigation of the efficacy of multicomponent drugs against heart failure[Pubmed: 30230922]
Endokrynologia PolskaEndothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension?…[Pubmed: 30359461]
International Journal of Peptide Research and TherapeuticsANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma[]
Van Veterinary JournalRelationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.[]
Acta Pharmaceutica Sinica BThe poly (ADP-ribosyl) ation of BRD4 mediated by PARP1 promoted pathological cardiac hypertrophy[34094834]
PeptidesAlarin moderated myocardial hypertrophy via inhibiting cyclic adenosine monophosphate/protein kinase A signaling pathway to attenuate autophagy[34624429]
PeptidesOsteocrin alleviates cardiac hypertrophy via attenuating oxidative stress[Pubmed:35218795]