粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay)
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal
（注：單次混測多因子不超過8個指標 ）
- 編號LMA705Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.1-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.033 ng/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA705Hu01 | 粘蛋白2(MUC2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA705Hu01 | 粘蛋白2(MUC2)多克隆抗體 | ICC/IF |
| LAA705Hu71 | 粘蛋白2(MUC2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA705Hu21 | 粘蛋白2(MUC2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA705Hu24 | 粘蛋白2(MUC2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA705Hu22 | 粘蛋白2(MUC2)單克隆抗體 | WB |
| MEA705Hu | 粘蛋白2(MUC2)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA705Hu | 粘蛋白2(MUC2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA705Hu | 粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA705Hu01 | 粘蛋白2(MUC2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.[PubMed: 20717734] |
| World J Gastroenterol. | Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats.[Pubmed:24627600] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis[PubMed: 26217323] |
| nutrition research and practice | Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway[PubMed: 26244071] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis[PubMed: 26172851] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization[doi:10.1513] |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Bio-protocol | Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA[10.21769:BioProtoc.2879] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease[Pubmed: 30573494] |
| Biomolecules | Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer[Pubmed: 30682816] |
| Cell Stem Cell | PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism[Pubmed: 31564549] |
| DIGESTIVE DISEASES AND SCIENCES | Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats[Pubmed: 32144600] |
| Scientific Reports | Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli[Pubmed: 32601325] |
| Biorxiv | Defective intestinal repair after short-term high fat diet due to loss of efferocytosis[] |
| J Cell Mol Med | Inhibition of cyclooxygenase?\2 enhanced intestinal epithelial homeostasis via suppressing |??\catenin signalling pathway in experimental liver fibrosis[34145945] |
| Cell Tissue Res | Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced?…[34370080] |