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軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Netrin 1 (Ntn1) ,etc. by FLIA (Flow Luminescence Immunoassay)

NTN1L; Epididymis tissue protein Li 131P

（注：單次混測多因子不超過8個指標 ）

  • 軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)97-104101
EDTA plasma(n=5)90-9894
heparin plasma(n=5)78-9084

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)80-101%80-99%95-102%86-101%
EDTA plasma(n=5)99-105%94-101%96-104%85-95%
heparin plasma(n=5)92-101%88-101%79-101%99-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號適用物種：Mus musculus (Mouse，小鼠)應用(僅供研究使用，不用于臨床診斷！)
RPB827Mu01軸突生長誘向因子1(Ntn1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB827Mu02軸突生長誘向因子1(Ntn1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB827Mu01軸突生長誘向因子1(Ntn1)活性蛋白Cell?culture;?Activity?Assays.
PAB827Mu02軸突生長誘向因子1(Ntn1)多克隆抗體WB; IHC; ICC; IP.
PAB827Mu01軸突生長誘向因子1(Ntn1)多克隆抗體WB; IHC; ICC; IP.
LAB827Mu81軸突生長誘向因子1(Ntn1)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
LAB827Mu71軸突生長誘向因子1(Ntn1)多克隆抗體(生物素標記)WB; IHC; ICC.
SEB827Mu軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB827Mu軸突生長誘向因子1(Ntn1)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
Transplantation ProceedingsNetrin-1: A Novel Universal Biomarker of Human Kidney Injury[PubMed: 20620466]
BiomarkersPlasma netrin-1 is a diagnostic biomarker of human cancers[PubMed: PMC3143477]
Journal of Maternal-Fetal and Neonatal MedicineCord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth[Informahealthcare: Source]
4Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity[Pubmed:24584118]
J Matern?Fetal?Neonatal Med.?Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth[Pubmed:24716747]
Renal failure.Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury[Pubmed:25154466]
Stem Cell Research & TherapyNetrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC)[articles:10.1186]
Oncotarget The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium.[pubmed:29156815]
Frontiers in?Cellular?NeuroscienceNeuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats[Pubmed:29375318]
Scientific ReportsNetrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor[Pubmed:29662125]
Family Practice and Palliative CareRelationship of serum netrin-1 levels with breast masses[Doi: 10.22391/fppc.467700]
JOURNAL OF BIOLOGICAL CHEMISTRYRole of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity[Pubmed: 30910812]
International Journal of Molecular?SciencesDownregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus[Pubmed: 30897795]
Cancer BiomarkSerum netrin-1 as a biomarker for colorectal cancer detection[Pubmed: 32474463]
Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos[]
Tuberk ToraksAssociation of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD[33853303]
Adv Healthc MaterConductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape[Pubmed:35670309]