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軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Netrin 1 (Ntn1) ,etc. by FLIA (Flow Luminescence Immunoassay)

NTN1L; Epididymis tissue protein Li 131P

（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）

  • 軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)97-104101
EDTA plasma(n=5)90-9894
heparin plasma(n=5)78-9084

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線(xiàn)性

在定值血清及血漿樣本內(nèi)加入適量的軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線(xiàn)性范圍即為稀釋后樣本中軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)80-101%80-99%95-102%86-101%
EDTA plasma(n=5)99-105%94-101%96-104%85-95%
heparin plasma(n=5)92-101%88-101%79-101%99-105%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈(zèng)品

相關(guān)產(chǎn)品

編號(hào)適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB827Mu01軸突生長(zhǎng)誘向因子1(Ntn1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB827Mu02軸突生長(zhǎng)誘向因子1(Ntn1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB827Mu01軸突生長(zhǎng)誘向因子1(Ntn1)活性蛋白Cell?culture;?Activity?Assays.
PAB827Mu02軸突生長(zhǎng)誘向因子1(Ntn1)多克隆抗體WB; IHC; ICC; IP.
PAB827Mu01軸突生長(zhǎng)誘向因子1(Ntn1)多克隆抗體WB; IHC; ICC; IP.
LAB827Mu81軸突生長(zhǎng)誘向因子1(Ntn1)多克隆抗體(異硫氰酸熒光素標(biāo)記)WB; IHC; ICC; IF.
LAB827Mu71軸突生長(zhǎng)誘向因子1(Ntn1)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
SEB827Mu軸突生長(zhǎng)誘向因子1(Ntn1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB827Mu軸突生長(zhǎng)誘向因子1(Ntn1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志參考文獻(xiàn)
Transplantation ProceedingsNetrin-1: A Novel Universal Biomarker of Human Kidney Injury[PubMed: 20620466]
BiomarkersPlasma netrin-1 is a diagnostic biomarker of human cancers[PubMed: PMC3143477]
Journal of Maternal-Fetal and Neonatal MedicineCord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth[Informahealthcare: Source]
4Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity[Pubmed:24584118]
J Matern?Fetal?Neonatal Med.?Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth[Pubmed:24716747]
Renal failure.Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury[Pubmed:25154466]
Stem Cell Research & TherapyNetrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC)[articles:10.1186]
Oncotarget The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium.[pubmed:29156815]
Frontiers in?Cellular?NeuroscienceNeuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats[Pubmed:29375318]
Scientific ReportsNetrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor[Pubmed:29662125]
Family Practice and Palliative CareRelationship of serum netrin-1 levels with breast masses[Doi: 10.22391/fppc.467700]
JOURNAL OF BIOLOGICAL CHEMISTRYRole of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity[Pubmed: 30910812]
International Journal of Molecular?SciencesDownregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus[Pubmed: 30897795]
Cancer BiomarkSerum netrin-1 as a biomarker for colorectal cancer detection[Pubmed: 32474463]
Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos[]
Tuberk ToraksAssociation of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD[33853303]
Adv Healthc MaterConductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape[Pubmed:35670309]