腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Renin (REN) ,etc. by FLIA (Flow Luminescence Immunoassay)
angiotensinogenase
（注：單次混測多因子不超過8個指標 ）
- 編號LMA889Rb
- 物種Oryctolagus cuniculus (Rabbit，兔)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標數(shù)7指標數(shù)6指標數(shù)5指標數(shù)4指標數(shù)3指標數(shù)2指標數(shù)1指標數(shù)
- 價格¥ 2562¥ 2660¥ 2808¥ 3005¥ 3202¥ 3497¥ 3941¥ 4926 添加到價格計算器 計算器
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-92 | 84 |
| EDTA plasma(n=5) | 90-101 | 96 |
| heparin plasma(n=5) | 86-101 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-104% | 78-104% | 86-104% | 78-93% |
| EDTA plasma(n=5) | 78-104% | 88-105% | 97-105% | 97-105% |
| heparin plasma(n=5) | 81-101% | 93-101% | 90-98% | 86-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA889Rb01 | 腎素(REN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA889Rb51 | 腎素(REN)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA889Rb21 | 腎素(REN)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA889Rb | 腎素(REN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA889Rb | 腎素(REN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Hypertension | Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the rat[PubMed: 21045727] |
| The Journal of Physiology | Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate [Physoc: 194241] |
| The Journal of Physiology | Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humans[PubMed: 20724370] |
| Pathobiology | Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells[Karger: 000330169] |
| Journal of Cardiovascular Pharmacology | The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic Plaques[LWW: 08000] |
| Kidney International | Multiple and large simple renal cysts are associated with prehypertension and hypertension.[Pubmed: 23389415] |
| Human Physiology | Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertension[Springer:Source] |
| Journal of Pharmacological Sciences | Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats[Pubmed:24366191] |
| Liver International | VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosis[Pubmed:24750552] |
| Gynecological Endocrinology | Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndrome[Pubmed:25200828] |
| Oxidative Medicine and Cellular Longevity | Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice[Pubmed:27200147] |
| PLoS One | Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria[Pubmed:27166674] |
| PLOS?ONE | Reduced?Expression?of the?Extracellular?Calcium-Sensing?Receptor?(CaSR) Is?Associated?withActivation?of the?Renin-Angiotensin?System?(RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.[pubmed:27391973] |
| Heart Lung Circ. | Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure.[pubmed:27555054] |
| European Journal of Pharmaceutical Sciences | Sacubitril/valsartan (LCZ696) Significantly Reduces Aldosterone and Increases cGMP Circulating Levels in a Canine Model of RAAS Activation[Doi: 10.1101/435560] |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats[] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice[33303990] |
| Hypertens Res | Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents[33568792] |
| Phosphorylation of ?21-integrin in juxtaglomerular cells helps control blood pressure during the progression of diabetic nephropathy[] | |
| Bedeutung des intrarenalen Renin-Angiotensin-Systems f¨1r die Entwicklung der chronischen Nierenkrankheit[] |