基質(zhì)金屬蛋白酶13(MMP13)重組蛋白
Recombinant Matrix Metalloproteinase 13 (MMP13)
CLG3; Collagenase 3
- 編號RPA099Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位分泌, 細(xì)胞外基質(zhì)
- 預(yù)測分子量22.3kDa
- 實(shí)際分子量25kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Glu103~Leu290 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)5.7
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA099Hu01 | 基質(zhì)金屬蛋白酶13(MMP13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA099Hu02 | 基質(zhì)金屬蛋白酶13(MMP13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA099Hu02 | 基質(zhì)金屬蛋白酶13(MMP13)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA099Hu01 | 基質(zhì)金屬蛋白酶13(MMP13)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA099Hu01 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA099Hu02 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA099Hu01 | 抗基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體 | Flow cytometry. |
| LAA099Hu71 | 基質(zhì)金屬蛋白酶13(MMP13)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA099Hu21 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Hu23 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Hu24 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Hu25 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Hu27 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Hu29 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA099Hu22 | 基質(zhì)金屬蛋白酶13(MMP13)單克隆抗體 | WB |
| HEA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA099Hu | 基質(zhì)金屬蛋白酶13(MMP13)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta[Wiley: source] |
| Arthritis & Rheumatism | Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice[Wiley: source] |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors[ScienceDirect: S0378111911007347] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
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| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655] |
| Arthritis & Rheumatol | Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis[Pubmed:24578232] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis[Pubmed:25448261] |
| Cellular Physiology and Biochemistry | Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering[Pubmed:25632940] |
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| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis[PubMed: 26578310] |
| PLoS One | Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression[PubMed: 26474296] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica[Pubmed:27157496] |
| Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds[pubmed:27821864] |
| International Journal of Contemporary Medical Research | Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma[uploads:7] |
| Scientific Reports | Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption.[pubmed:27640363] |
| Photodermatol Photoimmunol Photomed. | Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice[pubmed:27995657] |
| Molecular Medicine Reports | Icariin inhibits MMP?1, MMP?3 and MMP?13 expression through MAPK pathways in IL?1β?stimulated SW1353 chondrosarcoma cells[pubmed:28447732] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.[pubmed:28187731] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models[pubmed:27739057] |
| BMC Musculoskeletal Disorders | Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture[pubmed:29149841] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Alexandria Journal of Medicine | L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats[10.1016:j.ajme.2016.03.002] |
| National Journal of Integrated Research in Medicine | Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma.[AN=123116960] |
| Molecular?Medicine?Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits[Pubmed:29257344] |
| Journal of Applied Animal Research? | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis[10.1080:09712119.2017.1335208] |
| Osteoarthritis Cartilage | Coordinated existence of multiple gangliosides is required for cartilage metabolism[Pubmed: 30471358] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis[Pubmed: 30939166] |
| Pathobiology | Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma[Pubmed: 31129674] |
| Bone | DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion[Pubmed: 31283994] |
| Food & Function | A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese?…[] |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes[Pubmed: 32162384] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis[Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis[Pubmed: 31980722] |
| Nutrients | Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial[Pubmed: 31888255] |
| Nature Communications | Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13[Pubmed: 32807790] |
| Molecular Medicine Reports | MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression[Pubmed: 32705199] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs[] |
| Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model[] | |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs[] | |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice[33890588] |
| Nutrients | Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo?…[34444810] |
| Sci Rep | Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis[Pubmed:35091584] |