雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)
Mini Samples ELISA Kit for Estradiol (E2)
17B-Estradiol; Oestradiol; Beta-Estradiol
- 編號(hào)MEA461Ge
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍12.35-1,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于5.15pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價(jià)格¥ 2008¥ 2868¥ 12906¥ 24378¥ 200760
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-96 | 88 |
| EDTA plasma(n=5) | 87-94 | 90 |
| heparin plasma(n=5) | 98-105 | 101 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-92% | 83-97% | 81-104% | 93-101% |
| EDTA plasma(n=5) | 79-93% | 78-93% | 87-96% | 86-105% |
| heparin plasma(n=5) | 91-105% | 85-97% | 94-103% | 81-97% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL后，立即加入檢測(cè)溶液A25µL，
37°C孵育1小時(shí)；
3. 甩干，洗板3次；
4. 加檢測(cè)溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原(標(biāo)準(zhǔn)品或樣本)，待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過(guò)溫育和徹底洗滌后加入底物TMB顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測(cè)標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測(cè)物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
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| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA461Ge11 | 雌二醇(E2)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA461Ge21 | 雌二醇(E2)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA461Ge31 | 雌二醇(E2)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA461Ge01 | 雌二醇(E2)多克隆抗體 | ELISA |
| LAA461Ge71 | 雌二醇(E2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA461Ge81 | 雌二醇(E2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA461Ge26 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA461Ge21 | 雌二醇(E2)單克隆抗體 | IHC |
| MAA461Ge22 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA461Ge29 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA461Ge28 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA461Ge27 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA461Ge | 雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA461Ge | 雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA461Ge | 雌二醇(E2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA461Ge | 雌二醇(E2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA461Ge | 雌二醇(E2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA461Ge11 | 雌二醇(E2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| JOURNAL OF ETHNOPHARMACOLOGY | Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis[33422655] |
| JOURNAL OF ETHNOPHARMACOLOGY | BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-|êB pathways in aging mice induced by D-galactose[33508368] |
| Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice[] | |
| Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency[] | |
| Arch Gynecol Obstet | The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese?…[34417839] |
| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice[34730391] |
| Stem Cells Int | Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in?…[34733331] |
| Eur J Pharmacol | 17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice[34822791] |
| IBRO Neuroscience Reports | Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice[34746914] |
| Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice[Pubmed:35057459] |
| Nat Commun | TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice[Pubmed:35177657] |
| Appl Biochem Biotechnol | ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary[Pubmed:35138554] |
| Stem Cell Research & Therapy | G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and?…[] |
| Research Square | Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency[] |
